;Isoflavone aglycones have been reported to help in the prevention of cancer, cardiovascular diseases, osteoporosis, obesity . It plays an important role in human health and has been discussed for several years. Isoflavone aglycones can be produced from isoflavone glucosides. In this work, a fermentation process with soymilk which replaces traditional soybean as substrate has been performed. Besides, different light conditions will also has a strong connection with microbial growth and fermentation behavior.
In this work, we have explored the effects of light condition on bioconversion of isoflavone glucosides into isoflavone aglycones using Rhizopus oligosporus as well as develop strategies to enhance isoflavone aglycones production. Experimental results showed that at red light 200 lux condition performed the optimum cells growth, which was 38% higher than dark group. But at blue light condition, which light intensity was 100 lux, was more favorable for product yield based on biomass. It could perform 35% higher than the dark group. Therefore, two-stage shake flask fermentation by light control was conducted. Daidzein concentration using two-stage fermentation was 24.5µg/ml which corresponds to 26 % higher than single-light fermentation at red and blue light. On the other hand, Genisrein concentration using two-stage fermentation was 20.26µg/ml which corresponds to 15 % higher than single-light fermentation at red light.