| 摘要: | 本研究以磷脂質和膽固醇，作為微脂體的包覆材料，形成空心球狀的雙分子磷脂層膜，由於生物體的細胞膜結構也是雙分子磷脂層膜，因此與人體具有良好的相容性和生物可分解性，而球狀結構使其能作為藥物和抗氧化劑的載體，且能有效保護其內容物，在醫學、食品、保養品方面廣為大家所用。本研究使用脂質薄膜水合法製備蝦紅素微脂體溶液，將蝦紅素包覆於微脂體中，使其能保護蝦紅素不被溫度和光線分解，傳統上使用氯仿、甲醇和醚類作為合成上的溶劑，而本研究使用乙醇和天然食品作為其材料，在食安問題上較不會有疑慮。本研究首先改變不同比例的磷脂質和膽固醇來製備微脂體溶液，並將各樣品分別至於4 oC和25 oC放置60天，對其做安定性測試，分別在第7、14、30、60天時對其進行測試，利用紫外光-可見光近紅外光光譜儀觀察其濃度和包覆效果的變化，動態光散射分析儀做粒徑測試，並以光學顯微鏡對其外觀進行觀測，最後找出最佳的合成比例。
由於產品之後希望進行商業化生產，經由上述之實驗，找出最佳的包覆比例後，會進行劑量的放大測試，研究上先以放大5倍做測試，在劑量提升下，希望蝦紅素微脂體溶液仍保有小劑量時的效果，實驗上一樣會分別保存4 oC和25 oC環境，並對樣品做濃度、包覆率、粒徑和外觀的測試。
由本實驗結果得知，樣品須保存在4oC的條件下，且在磷脂質和膽固醇以4:1的條件下合成出來的微脂體具有最好的包覆效果(達92 %)和最小的粒徑(96.3 nm)，外觀上呈現一顆一顆圓形橘紅色，有效證明蝦紅素被包覆於微脂體中，而在放大規模的實驗中，得到與小劑量時類似的效果，在未來若能有效應用於商業化上，將具有良好發展潛力。
;In this study, phospholipids and cholesterol were used as coating materials for liposomes to form a hollow spherical bimolecular phospholipid layer membrane. Since the cell membrane structure of an organism is also a bimolecular phospholipid layer membrane, it has good compatibility with the human body and has high biodegradability. The spherical structure can be used as a carrier for drugs and antioxidants, and can effectively protect its contents. It is widely used in medicine, food, and maintenance products. In this study, a thin film hydration method was used to prepare astaxanthin liposome solution. Astaxanthin was encapsulated in the liposome to protect it from decomposition under high temperature and light. Traditionally, chloroform, methanol and ether were used as a solvent in synthesis. In this study ethanol and natural food were used, so there is no doubt on food safety. In this study, different ratios of phospholipids and cholesterol were used to prepare liposome solutions, and each sample was placed at 4 oC and 25 oC, respectively, for 60 days. The stability test was performed on days 7, 14, 30, and 60, respectively. Ultraviolet-visible near-infrared spectrometer (UV-vis) was used to determine its concentration and coating effect, dynamic light scattering analyzer (DLS) was used to do particle size test, and optical microscope (OM) was used to observe its appearance.
Since the product is expected to be commercialized afterwards, a scale-up test was conducted. In the research, the test was firstly performed at a magnification of 5 times. The product was stored at 4 oC and 25 oC, respectively, and then did the concentration, encapsulate efficiency., particle size and appearance tests.
The results show that the samples must be stored at 4 oC, and the liposomes synthesized under the condition of 4: 1 phospholipid and cholesterol had the best coating effect (up to 92%) and the smallest particles Diameter (96.3 nm). The appearance is round orange red, which can also effectively prove that astaxanthin is coated in liposomes. In the scaled-up test, the product was similar with that at the small scale. The method developed in this study has commercial potential. |
