通過將功能性奈米材料與生命系統整合而成的奈米生物雜化物已經成為橫跨材料工程和生物科學界的一項新興領域，人們嘗試透過結合功能性材料與生命系統期望改善適應環境壓力的能力、增強生物系統中現有的功能或實現全新的功能，其中新興孔洞金屬有機骨架材料 (MOFs)，因為可藉由調整金屬離子與有機配體組合的提供多樣選擇性，依需求設計，且具有一致的孔洞性質、高比表面積、優異的熱及化學穩定性、有催化功能的開放金屬位點等，因此近期有一系列著重在金屬有機骨架材料與生物系統結合的相關研究。 本研究先以類沸石咪唑骨架材料-8 (ZIF-8) 塗層於大腸桿菌表菌表面 (E. coli⊂ZIF-8)，並藉由抗生素來驗證文獻中曾提到ZIF-8塗層可能存在著缺陷，進而使抗生素會透過缺陷造成大腸桿菌的死亡，並進一步利用本實驗室於2015年發表於JACS利用類沸石咪唑骨架材料-90 (ZIF-90) 封裝酵素之技術，擴展到原核生物-大腸桿菌，形成微米級ZIF-90封裝大腸桿菌 (E. coli@ZIF-90) 來提供完整的保護，在經歷培養於含抗生素的環境後，移除材料的大腸桿菌依然可以回復其生長狀態，我們藉由掃描式電子顯微鏡及共聚焦顯微鏡來觀察兩者 (ZIF-8/ZIF-90) 封裝之差異，並嘗試藉由zeta電位之量測與紅外光譜儀的數據來了解造成兩者差異之原因，希望能將此一研究成果應用於生物系統之運輸與儲存、細胞固定化、生物傳感等領域。 ;Nanobiohybrids, synthesized by integrating functional nanomaterials with living systems, have emerged as a novel branch of research at the interface of materials engineering and biological science. Nanobiohybrids use synthetic nanomaterials to overcome harsh environmental problems and impart organisms with emergent properties. Among all materials, Metal-organic Frameworks (MOFs) is a novel material made from metal ions and organic linkers. MOFs have consistent pore properties, large surface area, thermo-chemical stability, catalyzing, etc through the adjustment of the precursors. Therefore, the development of the combination of Metal-organic frameworks and living systems is rapidly emerging. The prokaryotic living cell Escherichia coli (E. coli) for the first time is encapsulating into a single crystalline particle of Metal-organic Frameworks via de novo approach to create a novel biocomposite of “cyborgcells”. In the de novo approach, E. coli is entirely armored with single-crystalline ZIF-90 microporous in a single macro-size particle for the completely size-shielding protection, and therefore E. coli can be survived even lethal antibiotic treatment. By comparison with ZIF-8, the poly-crystalline multiple-nanoparticles are formed in surrounding on E. coli surface as their higher zeta potential differences, but with poor antibiotic protection. We hope that this model may pave the way to new avenues for the transportation and storage of living systems, cell immobilization, biosensing.
