複合式物理力的生物反應器自動化與控制設計;Multiplex physical stress bioreactor control and automation

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題名:	複合式物理力的生物反應器自動化與控制設計;Multiplex physical stress bioreactor control and automation
作者:	董新文;Dong, Hsin-Wen
貢獻者:	機械工程學系
關鍵詞:	生物反應器;控制;自動化;bioreactor;control;automation
日期:	2020-08-19
上傳時間:	2020-09-02 19:14:23 (UTC+8)
出版者:	國立中央大學
摘要:	本論文旨在低成本、易製備的基礎上探討生物反應器的製程與控制。在細胞培養上，我們常會使用Lab-on-chip創造仿生環境來在體外培養細胞以及觀察。大多數進行生物反應器的研究流於小尺度的觀察，而卻捨棄了批量生產細胞的可能性。同時，大量的微製程以及模擬生物活動所需的電路控制也對生物實驗者產生裝置製備方面的負擔。所以，適當的放大生物反應器的尺度使其兼顧觀察、採集等特性，仍然是一個問題。通常大多數的學者並未著墨於放大尺度以大量生產、降低成本、降低實驗者製造能力，因此本論文會就此方面進行相關探討。本文針對了一種進行機械與電刺激的生物反應器進行了改進，使它能夠讓使用者自主簡易的生產、組裝，而後能夠輕易地學習調整參數靈活地進行實驗。我們利用CNC铣床與鑽床進行流道層的製備，搭配PPy (Polypyrrole, 聚吡咯) coating PDMS材質的薄膜提供導電性用以電刺激細胞。再將拋棄式導電薄膜黏附於流道層上，利用抽氣的方式提供拉伸用以機械刺激細胞。同時，我們製作了一個整合電極的外殼固定整個裝置從而改善接合問題，以及提升可靠度。此外，還有一個用於自動化控制的控制盒。控制盒將用於以電磁閥控制抽氣幫浦調整對細胞的機械拉伸刺激頻率，以及控制固態繼電器產生脈衝訊號對細胞進行電刺激，此兩者都能對細胞在分化上產生了顯著的幫助。並且，複合刺激也證實了兩者可相輔相成，獲取更大的效益。而導入自動化控制對於使用者能夠更好的控制實驗參數，而在往後也提升了裝置的擴充性。 ;This thesis aims to discuss the process and control of bioreactor based on low cost and easy preparation. In cell-culture, we often use Lab-on-chip to culture cells in vitro. Most bioreactor research has focused on small-scale observations, but has abandoned the possibility of mass-culturing cells. At the same time, a lot of microfabrication processes and the circuit control required to simulate biological activities also need device preparation ability of biological experiment operators. Therefore, how to make the experiment operators easy to make is still a problem. Most scholars usually do not focus on low cost and reduce the experimenter′s manufacturing ability, so this paper will discuss this aspect. In this thesis, we improved a bioreactor for mechanical and electrical stimulation, so that it can allow users to produce and assemble it easily, and then they can easily learn and adjust parameters to experiment flexibly. We use a CNC milling machine and a drilling machine to prepare the flow channel layer, and a film made of PPy coating PDMS material provides conductivity to electrically stimulate cells. Then the film is bonded on the fluid layer, and the stretching is provided by pump to mechanically stimulate the cells. At the same time, we made a shell with electrodes to fix the whole device to avoid liquid leak and replace the function of traditional culture dishes. In addition, there is a control box for electromechanical control. The control box will be used to control the pump with an electromagnetic valve to adjust the mechanical stretch to stimulate the cells, and to use a pulse signal made by SSR (solid-state-relay) to electrical stimulate the cells. Moreover, the complex stimulation also confirmed that the two can help each other and gain greater benefits. The digital control of the device also allows they to adjust their own parameters needed, and it also remains device’s expandability in the future.
顯示於類別:	[機械工程研究所] 博碩士論文

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