| 摘要: | 多氯乙烯為我國主要有害人體之地下水汙染化合物，含氯有機物因其物化特性比水重之非水項液體(Densenon-aqueousphaseliquid,DNAPL)，一旦此類汙染物進入地下環境後，因其低黏滯性使其可在地表下快速移動，造成整治困難度高，其中三氯乙烯與氯乙烯均為公認人體致癌物質，因此多氯乙烯類的整治對於我國人民健康安全非常的重要。目前眾多多氯乙烯場址整治方式以生物整治為主，生物整治可細分為生物刺激法以及生物加強法，為了檢驗整治之功效，即時的動態生物監控貴為重要，該檢驗結果影響至後續整治工法之策略以及應變，但龐大的監測量導致整體工程費用居高不下或是利潤極少，且總體基因體菌相分析產生之雜訊以及龐大數據造成現地使用者不容易直觀應用數據。為突破此困境，本計畫將嘗試開發環境生物晶片以快速檢測脫氯菌群菌相變化以及整治成效。計畫為期三年，第一年著重於脫氯菌群探針設計以及晶片前期測試，第二年確校生物晶片之靈敏度以及準確度，並且小量生產晶片並最佳化分析流程與製程，第三年將應用於現地場址樣本，並與定量PCR以及總體基因體菌相分析進行比較，提出成本效益分析，並建立監測套件將產品商業化。本計畫之預期成果對於產業界具有高度的需求性，並且有助於我國脫氯整治技術能更上一層樓。 ;Polychlorinated ethylene, a kind of toxicant, is a common groundwater pollutant in Taiwan. The physicochemical properties of this pollutant is dense non-aqueous phase liquid (DNAPL) . Once it enters the underground environment, it moves quickly under the surface due to its low viscosity, which makes it difficult to be remediated. Among them, trichloroethylene (TCE) and vinyl chloride are recognized as human carcinogens. Therefore, the remediation of polyvinyl chloride is essential for the health of people. At present, many methods for remediation of polychlorinated ethylene sites are mainly focused on bioremediation. Bioremediation can be subdivided into biostimulation and bioaugmentation methods. In order to test the efficacy of biomediation, real-time dynamic biological monitoring is essential for monitoring the community of the potential bacteria for bioremediation via metagenome. However, the cost of metagenome is high, and the analysis of metagenome data is performed with bioinformatics, which could deter the use of metagenome in bioremediation. Therefore, our project will attempt to develop TCE-based genes microarray, a cost and timing- saving method, that can be efficiently tested for dechlorination bacteria and the effects of remediation. The three-year project will focus on dechlorination probe design and pre-sample testing in the first year, confirming the sensitivity and accuracy of the microarray. In the second year, we will produce microarray in small quantities and optimize the analytical process. The third year our developing microarray will be applied to pollutant water, whose data will be compared to the data of quantitative PCR and metagenomic analysis. Finally, after cost-benefit analysis, we hope to commercialize our microarray. The aim of this project is to provide an innovative technology which help a lot of companies in Taiwan, to devise a bioremediation strategy based on the kinetic changes of bacterial community. Speedy, efficiency, and cost-saving are our hot spots for developing microarray. |
