複雜性的創傷如慢性傷口，因癒合緩慢，若持續未完善處理，一旦遭受細菌感染，就可能造成蜂窩性組織炎，更可能引發敗血症，因此需開發多功能的傷口敷料來因應其狀況，本研究選用褐藻酸鈉與聚己內酯為靜電紡絲的高分子材料，褐藻酸鈉具親水性可為傷口提供潮濕環境，聚己內酯則因其有良好的生物相容性以促進細胞貼附，除此之外，我們將奈米銀引入到聚己內酯纖維中使其抑制細菌的生長，以達到抗菌效果，並藉由將血小板衍生生長因子（platelet-derived growth factor B，PDGFB）與聚乙烯亞胺（PEI）結合為帶正電奈米顆粒，使其可與帶負電的褐藻酸鈉纖維做結合，再藉由原位轉染的方式使細胞可以表達PDGFB，最後藉由褐藻酸鈉與聚己內酯纖維各自不同的特性來調整不同比例的褐藻酸鈉與聚己內酯纖維，以達到擁有多功能效果的傷口敷料。;Wound healing is an important issue in clinical situation. For example, chronic wounds are slow to heal. The prolonged inflammatory phase in chronic wounds may result in not only an immature granulation tissue but also a parallel reduction of wound tensile strength, which always eventually cause amputations. Therefore, it is necessary to develop a multifunctional wound dressing to promote tissue regeneration. In this study, alginate and polycaprolactone (PCL) fibers are investigated as materials for wound dressing preparation. Alginate is a hydrophilic material and can provide a moist environment in wound site. Polycaprolactone has good biocompatibility to promote cell adhesion. In addition, we introduced the silver nanoparticles into polycaprolactone fibers to inhibit the growth of bacteria. To promote tissue regeneration, plasmid DNA encoding platelet-derived growth factor B (PDGFB) was complexed with polyethyleneimine (PEI) as positively charged nanoparticles to adsorb on negatively charged sodium alginate fibers. Therefore, cells contact fibers can thus be transfected to express PDGFB. Through coelectrospinning, alginate and PCL were prepared as composite fibers, and their properties can be manipulated by adjusting fiber ratios to achieve a versatile wound dressing.
