以毛豆為原料萃取煙醯胺單核?酸之研究;Extraction of Nicotinamide Mononucleotide from Edamame

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题名:	以毛豆為原料萃取煙醯胺單核?酸之研究;Extraction of Nicotinamide Mononucleotide from Edamame
作者:	曾馨儀;Tseng, Hsin-I
贡献者:	化學工程與材料工程學系
关键词:	毛豆;煙胺醯單核?酸;萃取;edamame;nicotinamide mononucleotide;extraction
日期:	2021-07-07
上传时间:	2021-12-07 11:13:45 (UTC+8)
出版者:	國立中央大學
摘要:	保健食品原料來源主要由動物、植物或微生物而來，而本實驗選擇植物來源做為原料。萃取有許多方法如蒸餾法、微波輔助萃取法、超臨界流體萃取等。純化方法也有許多種，如層析法、離子交換法、超臨界流體技術亦能作為純化方法。煙醯胺單核?酸(NMN)是衍生自核糖及煙醯胺的一種核?酸，NMN是由體內的B群維生素製成的，是在所有生物體中天然存在的一種分子，能防止肥胖、抗衰老、保護神經、防止心血管疾病等優點。本實驗使用煙醯胺單核?酸含量最高之食物－毛豆作為原料，透過天然食物萃取出煙醯胺單核?酸並純化，可作為新興保健食品。選用丙酮及水作為萃取煙醯胺單核?酸之溶劑，並使用真空烘箱將毛豆萃取液做濃縮。結果顯示，利用水作為溶劑較易溶出煙醯胺單核?酸。使用穿透式電子顯微鏡(TEM)觀察到煙醯胺單核?酸大小約為50-70奈米。利用紫外光-可見光分光光譜儀(UV-Vis)顯示出煙醯胺單核?酸之吸收波峰在290 nm。最後用薄層分析法(TLC)將純煙醯胺單核?酸及毛豆萃取液做比較，確認毛豆萃取液中成功萃取出煙醯胺單核?酸。 ;The raw material of health food is mainly from animal, plant or microorganism, and this experiment chose plant source as the raw material. There are many methods of extraction such as distillation, microwave-assisted extraction, supercritical fluid extraction, etc. There are also many purification methods, such as chromatography, ion exchange, supercritical fluid technology can also be used as purification methods. 　　Nicotinamide mononucleotide (NMN) is a nucleotide derived from ribose and nicotinamide. NMN is made from the body′s B-complex vitamins, a naturally occurring molecule in all living organisms, and can prevent obesity, anti-aging, neuroprotection, cardiovascular disease, and other benefits. In this study, the food with the highest content of nicotinamide mononucleotide, edamame, was used as the raw material. Extraction and purification of nicotinamide mononucleotides from natural food can be used as a new health food. Acetone and water were used as solvents to extract nicotinamide mononucleotides, and the extract was concentrated in a vacuum oven. The results show that the nicotinamide mononucleotides were more easily extracted by using water as the solvent than acetone. The size of nicotinamide mononucleotide was observed to be 50-70 nm by using transmission electron microscope. The absorption peak of nicotinamide mononucleotide was shown to be around 290 nm by UV-Vis spectroscopy. Finally, the pure nicotinamide mononucleotide was compared with edamame extract by thin layer analysis (TLC), and it was confirmed that nicotinamide mononucleotide was successfully extracted from edamame extract.
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