開發高效本土脫氯菌劑及優化現地生物加強傳輸工法：現地模場試驗

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題名:	開發高效本土脫氯菌劑及優化現地生物加強傳輸工法：現地模場試驗
作者:	陳師慶;董天行
貢獻者:	國立中央大學生命科學系
關鍵詞:	脫鹵球菌;生物加強;生物整治;三氯乙烯;完全脫氯;;Dehalococcoides;Bioaugmentation;Bioremediation;Trichloroethene;Completed dechlorination
日期:	2023-03-08
上傳時間:	2023-03-08 15:11:10 (UTC+8)
出版者:	行政院環境保護署
摘要:	含氯有機物如三氯乙烯為難降解之污染物，常污染我國地下水並且整治難度高，污染場址的解列率僅不到兩成。目前現地整治遇到最大的瓶頸是業者多使用化學藥劑進行生物整治，整治最終導致毒性更強並能致癌的氯乙烯(Vinyl chloride)的累積，這個因素常常讓一個場地的整治失敗。本團隊透過過去與產業端合作，收集全台含氯有機物污染之地下水，進一步培養台灣本土脫氯菌群，使用本團隊獨有的脫鹵球菌(Dehalococcoides)培養平台，我們成功地找到數個菌群可以快速且不易產生二次污染如氯乙烯的菌群，用以開發創新本土菌劑生物強化(Bioaugmentation)整治工法。本研究第一年度，透過本實驗室從現地模場建立的厭氧脫氯菌群資料庫中，去開發應用於現地的脫氯菌劑，我們將會在這個計畫中去使用發酵槽開發量產製程，使得菌量達可以達應用於現地整治之標準，同時確立脫氯菌劑的降解效能。第二年度本研究將以進入現地模場試驗，優化主動式生物加強工法，以雙環塞灌注工法提升菌劑灌注之影響範圍及精確度，找到工法實施最佳參數及流程，有效提升菌劑之利用。使用菌劑可以最大化提升生物基質之功效，更能降低生物基質的使用成本並且縮短整治工時，同時，菌劑可以有效解決氯乙烯累積問題，預期使用脫氯菌群生物加強法可以有效改善台灣含氯有機物之整治瓶頸。 ;Organic halide compounds such as trichloroethyene are refractory pollutants, which often pollute the groundwater in our country and are difficult to remediate. The decommissioning rate of polluted sites is only less than 20%. At present, the biggest bottleneck encountered in remediation is that operators often use chemical agents for biological remediation. Remediation eventually leads to the accumulation of vinyl chloride, which is more toxic and carcinogenic. This factor often makes the remediation of a site fail. Through cooperation with the industry in the past, our team has collected groundwater contaminated by chlorinated organic compounds throughout Taiwan, and further cultivated Taiwan's native dechlorination bacteria. Using our unique Dehalococcoides culture platform, we have successfully found several consortia. These consortia can quickly and less produce secondary pollution such as vinyl chloride, to develop innovative novel bioaugmentation strategy. In the first year of this project, through the laboratory of the anaerobic dechlorination bacteria database established by the on-site model field, we will develop the dechlorination consortia used in the field. We will use the fermentation tank in this project to develop. The production process enables the consortia to reach the standard that can be applied to on-site remediation, and at the same time establishes the degradation efficiency of the dechlorination bacteria agent. In the second year, this research will enter the field test in the field, optimize the active bio-enhancement method, use the double packer injection method to improve the influence range and accuracy of the inoculum infusion, find the best parameters and procedures for the implementation of the construction method, and effectively improve the inoculum's performance. use. The use of consortia agent can maximize the efficacy of biological substrates, reduce the cost of using biological substrates and shorten the remediation time. At the same time, consortia agent can effectively solve the problem of vinyl chloride accumulation. It is expected that the use of dechlorination consortia bioaugmentation method can effectively improve remediation bottleneck of organic halide contamination in Taiwan.
關聯:	財團法人國家實驗研究院科技政策研究與資訊中心
顯示於類別:	[生命科學系] 研究計畫

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