| 摘要: | 生物列印在這幾年在蓬勃發展，對於列印人體組織，像是皮膚、血管及支架等，已有不少的成果。但這些列印出來的成品，往往會因為積料問題，導致整體列印出來的形狀高度有些許誤差，甚至外圍的輪廓也因為積料，在接近邊緣處的孔洞會因為積料而被覆蓋，導致整體看起來是黏合在一起的，這可能會使得列印出來的生物支架尺寸不符合需求。目前大多使用的生物列印機台，主要都是龍門式的，所以進行轉角處的列印時，會經歷兩軸的加減速，而積料問題往往就是轉角處最為嚴重。
為了嘗試改善轉角積料問題，本研究提出了三種不同的列印策略，分別為加速策略、平滑化策略以及關料策略，並且使用殼聚醣搭配低溫列印機台，列印出不同角度的轉角以及不同間距的支架，並觀察這些支架的積料狀況。為了能夠更加數值化的分析轉角狀況，還對實驗所拍攝的影像進行處理，以面積及線徑的分析方法，比較各個策略列印結果的優劣。
從影像分析結果可以發現，能夠最多改善積料面積的策略為加速策略，不管是任何角度還是任何間距都能有效改善，不過會造成經過轉角後股線震盪;平滑化策略對於不同角度也都有一定程度的減少積料，不過相比加速列印，減少的幅度相對較少，還會一定程度造成轉角處的位置偏移;關料策略則是有嚴重的延遲現象，使得積料狀況幾乎沒有任何改善。;Bioprinting has been thriving in the past few years, and there have been many achievements
in printing human tissues, such as skin, blood vessels, and stents. However, these printed
products often have some errors in the overall shape and height due to the accumulation of material, and even the contours are covered by the holes near the edges due to the accumulation of material, resulting in the overall appearance of bonding together, which may make the printed scaffolds not meet the size requirements. Most of the bioprinting machines used today are mainly gantry type, so when printing in the corners, they will experience acceleration and deceleration of both axes, and the accumulation problem is often the most serious in the corners.
To improve the corner accumulation problem, three different printing strategies were proposed, namely acceleration strategy, smoothing strategy, and closing material strategy. Chitosan was used to print large-pitch and small-pitch scaffolds by low-temperature printing machine and observe the accumulation of these scaffolds. To analyze the corner conditions more numerically, the images of scaffolds were image processed and the advantages and disadvantages of
each strategy were compared by analyzing the area and linewidth.
From the results of the image analysis, we found that the acceleration strategy can improve the accumulation area most, which is effective at any angle and any pitch, but causes the strands to oscillate after the corner; The smoothing strategy also reduces the accumulation to a certain extent for different angles, but the reduction is relatively small compared to the acceleration strategy, and it also causes the position shift at the corner; The closing material strategy has serious delays, making little or no improvement in the accumulation of corners. |
