開發鈉鉀離子交換系統應用於體外耳蝸前驅細胞分化;To establish Sodium/Potassium Exchange System for In-vitro Cochlear Progenitor Cell Differentiation

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题名:	開發鈉鉀離子交換系統應用於體外耳蝸前驅細胞分化;To establish Sodium/Potassium Exchange System for In-vitro Cochlear Progenitor Cell Differentiation
作者:	丁郁軒;Ting, Yu-Hsuan
贡献者:	生物醫學工程研究所
关键词:	聽損;毛細胞;內淋巴液;海藻酸鉀;hearing loss;hair cell;endolymph;potassium alginate
日期:	2023-02-01
上传时间:	2024-09-19 15:11:19 (UTC+8)
出版者:	國立中央大學
摘要:	聽力損失是一種常見病，會給生活帶來諸多不便。導致聽力損傷的因素有很多，如遺傳缺陷、環境噪聲、耳毒性藥物和衰老的影響。隨著人口老齡化問題，聽力損失人數將從2019年的1.5億增加到2050年的2.5億。聽力損失可分為兩種類型。第一種是傳導性聽力損失，通常是由於耳道內有異物阻塞或中耳腔積液所致。臨床上常採用手術治療或助聽器輔助來放大聲音訊號。另一種是感音神經性聽力損失（SNHL），多數是由於毛細胞損傷後無法再生，導致神經信號無法進入大腦，目前臨床上常見的治療方法是植入人工電子耳，但是成本很高而且不能根治。因此，引入細胞治療聽力損失是一種有潛力的方法。從胚胎發育的角度，我們知道在毛細胞分化的早期，內淋巴液從高鈉緩慢地變為高鉀。本研究的目的是利用海藻酸鉀加入氯化鍶交聯形成具有半透膜的K-beads。利用鈉鉀離子對海藻酸鹽的親和力不同，使培養基中的鈉離子被海藻酸鹽吸附。因此，K-beads 可以釋放鉀離子並減少鈉離子，從而創造動態離子調節，形成毛細胞分化的體外微環境。我們成功開發了一種體外動態離子調節系統，用於模擬體內內淋巴微環境變化。;Hearing loss is a common disease that can cause many inconveniences in life. There are many factors that can cause hearing loss, such as genetic defects, environmental noise, ototoxic drugs, and the effects of aging. With the ageing population problem, the number of people with hearing loss will increase from 150 million in 2019 to 250 million in 2050.Hearing loss can be divided into two types. The first is conductive hearing loss, which is usually caused by foreign body obstruction in the ear canal or fluid accumulation in the middle ear cavity. Clinically, surgical treatment or hearing aid assistance is often used to amplify sound signal. The other one is sensorineural hearing loss (SNHL), most of which is due to the damage of hair cells without regeneration, resulting in the inability of nerve signals to enter the brain At present, the common clinical treatment is to place the cochlear implant, but the cost is very high but cannot cure. Therefore, introducing cell therapy for hearing loss is a potential method. From the point of view of embryonic development, we knew the endolymphatic fluid changes from high sodium to high potassium slowly during the early stage of hair cell differentiation. The aim of the study, we use potassium alginate to add strontium chloride to cross-link to form K-beads with a semi-permeable membrane. Consequently, the K-beads can release potassium ions and reduce sodium ions to create dynamic ion regulation forming in-vitro microenvironment for hair cell differentiation. In summary, we successfully developed in-vitro dynamic ion regulation system for mimicking in-vivo endolymphatic microenvironment.
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