植物乳桿菌中黃素單核苷酸介導的電通過 I 型拼貼增強細菌對腸細胞的粘附;Flavin mononucleotide-mediated electricity in Lactobacillus plantarum enhances bacterial adhesion to intestinal cells via type I collagen

NCUIR > college of Health Sciences and Technology > Institute of Biomedical Engineering > Electronic Thesis & Dissertation > Item 987654321/92137

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/92137

Title:	植物乳桿菌中黃素單核苷酸介導的電通過 I 型拼貼增強細菌對腸細胞的粘附;Flavin mononucleotide-mediated electricity in Lactobacillus plantarum enhances bacterial adhesion to intestinal cells via type I collagen
Authors:	班筑雅;Ganzorig, Binderiya
Contributors:	生醫科學與工程學系
Keywords:	乳酸菌植物;細胞外電子轉移 (EET);電;黃素單核苷酸 (FMN);細胞粘附;I型膠原蛋白;L. plantarum;extracellular electron transfer (EET);electricity;flavin mononucleotide (FMN);cell adhesion;type I collagen
Date:	2023-06-19
Issue Date:	2024-09-19 15:19:54 (UTC+8)
Publisher:	國立中央大學
Abstract:	產生黃素單核苷酸 (FMN) 的細菌如何粘附在宿主腸道上的機制尚不清楚。為了解決這個問題，從蒙古發酵的 Airag (L. plantarum MA) 中分離出的革蘭氏陽性菌植物乳桿菌被用作模型微生物，它們的產電特性首先通過它們顯著表達 II 型 NADH:醌氧化還原酶來鑑定。植物乳桿菌 MA 的電活動後來被證明是通過基於黃素單核苷酸(FMN) 的細胞外電子轉移進行的，這在很大程度上取決於培養基中碳源的可用性。我們的研究結果表明，當 L. plantarum MA 在乳果糖存在的情況下培養 24 小時時，它可以產生大約 15 µM 的 FMN，FMN 是發電必不可少的電子供體之一。我們還表明，植物乳桿菌 MA 的電活動可以刺激微生物在 Caco-2 細胞和小鼠盲腸上的粘附和定植。這種改善的依從性是由於在用植物乳桿菌 MA 處理後腸上皮中 I 型膠原蛋白的表達增加。這項研究揭示了 L. plantarum MA 的產電活性機制，並展示了細菌如何利用電來改變腸道組織中的蛋白質生產以提高粘附性。;The mechanism is yet unknown that how flavin mononucleotide (FMN)-producing bacteria adhere to the host gut. In order to address this issue, Gram positive bacteria Lactobacillus plantarum isolated from Mongolian fermented Airag (L. plantarum MA) were used as model microbes and their electrogenic properties were first identified by their significant expression of type II NADH:quinone oxidoreductase. The electrical activity of L. plantarum MA was later demonstrated to be carried out by flavin mononucleotide (FMN)-based extracellular electron transfer, which was significantly dependent on the availability of a carbon source in the medium. Our findings reveal that when L. plantarum MA is cultivated in the presence of lactulose for 24 hours, it can produce around 15 µM of FMN, one of the essential electron donors for power generation. We also show that the electrical activity of L. plantarum MA can stimulate microbial adherence and colonization onto Caco-2 cells and mouse cecum. This improved adherence was due to increased expression of type I collagen in the intestinal epithelium after treatment with L. plantarum MA. This study unveils the mechanism of L. plantarum MA′s electrogenic activity and shows how the bacteria use electricity to alter protein production in gut tissue for improved adherence
Appears in Collections:	[Institute of Biomedical Engineering] Electronic Thesis & Dissertation

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