本研究針對不同腫瘤細胞接種方法，在三維明膠/羥基磷灰石/戊二醛（GHG）支架中所形成腫瘤仿體。利用被動接種的蓋壓法，以及主動接種的培養液注射法和明膠注射法將細胞植入支架中，並通過磁共振成像（MRI）觀察腫瘤組織的生長情況。結果顯示，蓋壓法會導致細胞主要集中於細胞液接觸面；培養液注射法中，細胞能分布於支架的注射上層與注射層，而注射下層的細胞液可能因重力作用而流失。明膠注射法中，觀察到支架的注射上層、注射層與注射下層的孔洞內皆有細胞被明膠包覆，並推測黏性的明膠能將細胞維持在孔洞內進行懸浮生長。在14天的培養後，三種接種方法在表層均能培養出緻密的組織團塊，其中明膠注射法不僅可以增加在組織團塊數，也能促進在支架縱向的分布。在MRI觀察中，影像解析度的限制為83 μm，因此無法成像較小的腫瘤組織或孔洞，但MRI能對腫瘤組織在支架的分布進行大尺度的觀察。此外，MRI影像能進行三維重建，但支架結構與腫瘤組織的訊號存在重疊，導致兩者無法清晰區分。未來研究可考慮對MRI影像進行優化處理，並對仿體進行連續觀察。;This study investigates the impact of different cell seeding methods on the formation of bionic tumors in a three-dimensional GHG（gelatin/hydroxyapatite/glutaraldehyde） scaffold. Three seeding methods were evaluated including passive seeding by capping and active seeding via culture medium injection and gelatin injection. Capping method allowed cell suspension passively absorbed to the scaffold, leading to cell primarily concentrated on the cell suspension-contacting surface. In the culture medium injection method, cells were distributed in the injection layer and its upper. Cell was unfindable in the region below the injection layer due to possible lost through gravity. In contrast, with the gelatin injection method, cells were observed encapsulated by gelatin within the pores not only in the injection layer but also its upper and lower regions, suggesting that gelatin′s supportive properties enabled cell suspended growth within the pores. After cultivation for 2 weeks, all seeding methods resulted in the formation of dense tissue, with a significant increase in vertical growth area observed in the gelatin injection method, indicating that the suspension culture in gelatin could promote tissue growth in the vertical dimension. These bionic tumor were monitored using magnetic resonance imaging（MRI）analysis.Due to the resolution limitation of 83 μm of MRI, it was difficult to image smaller tumor tissues or pores within the scaffold. However, MRI could conduct large-scale observations of the distribution of tumor tissue in the scaffold. The 3D reconstruction results of MRI showed that the signals of the scaffold structure and the tumor tissue overlaped, preventing clear distinction between the two. Future research could focus on optimizing MRI imaging and continuously monitoring tissue growth in the scaffold.
