以擠製冷卻成型法結合相分離法製作神經再生用多孔性導管

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姓名

韓士瑞(Shih-jui Han) 查詢紙本館藏

畢業系所

機械工程學系

論文名稱

以擠製冷卻成型法結合相分離法製作神經再生用多孔性導管
(The procedure combines extrusion freezing modeling to form nerve conduits and phase separation to generate porous conduits.)

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摘要(中)

組織工程用神經導管提供一個橋接神經斷傷兩端的方式，不會造成斷傷兩端神經的張力，創造一個符合神經細胞生長的人為環境，同時可選擇使用生物相容性良好的生醫材料當做製作神經導管材料。
本研究選擇的材料為聚乳酸(PLA)及聚乳酸-聚甘醇酸共聚物(PLGA)兩種目前組織工程主要的人工合成可降解生醫材料，利用擠製冷卻成型法結合熱誘導式相分離法和濕式相分離法，靠著升溫程序對時間的變化程序來製作多孔性的神經導管，討論不同濃度和幾何尺寸對微孔洞分佈和孔洞大小、機械性質、含水率、孔隙率等影響，並依電子顯微影像來觀察神經導管外壁面、橫截面、內壁面，發現神經導管微孔洞成魚骨狀分佈，及孔洞分佈趨勢。由材料試驗機中測得其機械性質中抗拉強度、抗彎強度、撕裂強度，會隨著高分子材料濃度越高或導管外徑越大其值越大，而神經導管伸長量、應變值、楊氏係數卻是反之。而含水率與孔隙率的測試結果發現隨著材料濃度越高其值越小，但導管外徑大小不會影響含水率和孔隙率的高低值。

摘要(英)

Porous nerve conduits are generally made by nontoxic biomaterials. It provides a bridge to connect the two ends of broken nerves and also an artificial environment for nerve regeneration.
In this research, a method of using PLGA and PLA biomaterials to fabricate nerve conduits is introduced. The procedure combines extrusion freezing modeling to form conduits, which then go through wet immersion phase separation and thermally induced phase separation to generate porous conduits. The properties of the conduit such as pore distribution, pore size, tensile stress and strain, water content, and porosity are tested and analyzed with respect to the concentration and dimension of the conduit. The inner surface, outer surface, and cross sections of the conduit are also scanned by SEM to find their pore sizes.
The experimental results show that tensile strength and fracture strength are direct proportion to the concentration and diameter of the conduit, while elongation, strain, and Young’s modulus are inverse proportion to them. Moreover, water content and porosity are direct proportion to the concentration of the biomaterial.

關鍵字(中)

★ 組織工程
★ 生醫材料
★ 相分離
★ 神經導管

關鍵字(英)

★ Tissue engineering
★ Biomaterial
★ Phase Separation
★ Nerve Conduits

論文目次

摘要 I
Abstract II
目錄 III
表目錄 VII
圖目錄 VIII
第一章緒論 1
第二章文獻回顧 3
2-1 神經系統介紹 3
2-1-1周邊神經的結構 3
2-1-2 周邊神經損傷分類 3
2-1-2-1 Seddon 分類法 3
2-1-2-2 Sunderland 分類法 4
2-2 生醫材料 4
2-2-1生醫材料用途 4
2-2-2組織工程用對生醫材料的要求 4
2-2-2 PGA、PLA、PLGA的介紹 6
2-3 組織工程神經導管 7
2-3-1神經導管之材質 8
2-3-2神經導管支架製備方法 9
2-3-2-1 電紡織法 9
2-3-2-2 擠製成型及鹽析法 9
2-3-2-3圓筒編織法及氣泡成型法 10
2-3-2-4黏度滾製法 10
2-4相分離的介紹 11
2-4-1相分離熱力學 11
2-4-2 相分離動力學 14
2-4-2-1 Desolvation 14
2-4-2-2 Demixing 14
2-4-2-3 Phase Transform(Coarsening) 15
第三章材料與方法 18
3-1 實驗材料 18
3-2 實驗儀器與器材設備 18
3-2-1神經導管的製備 18
3-2-2 神經導管的型態觀察 19
3-2-3神經導管機械性質測試 20
3-2-4 神經導管的含水率測試 20
3-2-5神經導管的孔隙率測試 20
3-3 實驗方法 20
3-3-1 神經導管的製備 20
3-3-2神經導管型態觀察 23
3-3-3神經導管機械性質靜態拉伸測定 24
3-3-4神經導管含水率測定 24
3-3-5神經導管孔隙率測定 25
第四章結果與討論 26
4-1 升溫程序對神經導管成型機制的影響 26
4-1-2 後處理 41
4-2材料、溶劑與非溶劑的組合對形成中空通道的關係 42
4-3 神經導管觀察 43
4-3-1神經導管巨觀結構型態觀察 43
4-3-2神經導管顯微結構及孔隙分布觀察 46
4-3-2-1 微孔洞大小 47
4-3-2-2管壁厚度 48
4-4 神經導管機械性質靜態拉伸測定 49
4-5 神經導管含水率與孔隙率測試 57
第五章結論與未來展望 67
5-1 結論 67
5-2 未來展望 67
參考文獻 69
附錄 74

參考文獻

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指導教授

曾清秀(Ching-Shiow Teseng)

審核日期

2007-10-11

推文