| 摘要(英) |
Behavior is driven by environmental stimulation that triggers brain activity with lots of
molecular mechanisms. The process of learning and memory contains stimulation-induced
changes that happen in the synaptic connections between neurons in the hippocampus, which
plays a pivotal role in the formation of long term somatic and episodic memories. By Morris
water maze experiment and RNA analysis, we show that some micro-RNAs (miRNA) are
expressed differently between learned and unlearned mice. One of the miRNAs, miR-466f-3p,
which belongs to post transcriptional regulator, is abundantly express in brain and generated in
the intron-10 of Sfmbt2 gene. However, the mRNA expression of Sfmbt2 is significantly
different from miR-466f-3p. Inhibition of epigenetic modification, such as DNA methylation,
can affect gene expression. Treatment of 5’Aza-dC, a methyltransferase inhibitor, to the
hippocampal primary culture neuron causes Bdnf, Nrf2, Sfmbt2 mRNA and miR466f-3p
expression increased. However, the methylation pattern in the promoter region of the miR-466f
cluster is not significantly different between learned and unlearned mice. The evidence show
that the changes of DNA methylation under the stress of the Morris water maze indirectly
participate in the regulating of miR-466f-3p induction in the hippocampus-dependent spatial
learning and memory formation.
Keywords: Epigenetic, mi-RNAs induction, Methylation |
| 參考文獻 |
REFERENCE
[1] Nunez J. (2008). Morris Water Maze Experiment. DOI: 10.3791/897. URL:
http://www.jove.com/index/Details.stp?ID=897
[2] Vorhees CV and Williams MT. (2006). Morris water maze: procedures for assessing
spatial and related forms of learning and memory. Nat Protoc. 2006; 1(2): 848–858.
doi:10.1038/nprot.2006.116.
[3] Barnhart CD., Yang D., and Lein PJ. (2015). Using the Morris Water Maze to Assess
Spatial Learning and Memory in Weanling Mice. PLOS ONE|
DOI:10.1371/journal.pone.0124521
[4] Inoue et al., (2017). The Rodent-Specific MicroRNA Cluster within the Sfmbt2 Gene Is
Imprinted and Essential for Placental Development. Cell Reports 19, 949–956.
http://dx.doi.org/10.1016/j.celrep.2017.04.018
[5] https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=353282
[6] http://www.informatics.jax.org/marker/MGI:2447794
[7] O′Brien J., Hayder H., Zayed Y., and Peng C. (2007). Overview of MicroRNA
Biogenesis, Mechanisms of Actions, and Circulation. Front Endocrinol (Lausanne).
Doi: 10.3389/fendo.2018.00402
[8] Day JJ and Sweatt JD. (2011). DNA methylation and Memory Formation. Nat
Neurosci.2010 Nov; 13(11): 1319–1323. doi: 10.1038/nn.2666 P
[9] Yang X., Shao X., Gao L. and Zhang S. (2016). Comparative DNA methylation analysis
to decipher common and cell type-specific patterns among multiple cell types. Brief
Funct Genomics. 2016 Nov;15(6):399-407. Epub 2016 Apr 23.
[10] Ptak C., BScH., Petronis A. (2010). Basic research I Epigenetic approaches to
psychiatric disorders. Dialogues Clin Neurosci. 2010;12(1):25-35.
[11] Whalley K. (2007). Dynamic DNA methylation. Nature Reviews.
Neuroscience volume8, page323 (2007)
[12] Zhang Z et al., (2016). Effects of 5-Aza-20-deoxycytidine on expression of PP1g in
learning and memory. Biomedicine & Pharmacotherapy 84 (2016) 277–283
[13] Lister R et al., (2013). Global Epigenomic Reconfiguration During Mammalian Brain
Development. Science. 2013 August 9; 341(6146): 1237905–1237905
[14] Christman JK. (2002). 5-Azacytidine and 5-aza-2′-deoxycytidine as inhibitors of DNA
23
methylation: mechanistic studies and their implications for cancer therapy. Oncogene
(2002) 21, 5483 – 5495
[15] Seelan RS et al., (2018). Effects of 5-Aza-2′-deoxycytidine (decitabine) on gene
expression. Drug Metab Rev. 2018 May;50(2):193-207. doi:
10.1080/03602532.2018.1437446.
[16] Ovkvist CL et al., (2016). DNA methylation in human epigenomes depends on local
topology of CpG sites. Nucleic Acids Research, 2016, Vol. 44, No. 11 5123–5132. Doi:
10.1093/nar/gkw124
[17] Darst RP. (2010). Bisulfite Sequencing of DNA. Curr Protoc Mol Biol. 2010 July;
CHAPTER: Unit–7.917. Doi:10.1002/0471142727.mb0709s91
[18] Li Y et al., (2019). DNA methylation, microRNA expression profiles and their
relationships with transcriptome in grass-fed and grain-fed Angus Cattle rumen tissue.
CC-BY 4.0 International license. doi: http://dx.doi.org/10.1101/581421
[19] Li Y and Tollefsbol TO. (2011). DNA methylation detection: Bisulfite genomic
sequencing Analysis. Methods Mol Biol. 2011; 791: 11–21.
doi:10.1007/978-1-61779-316-5_2.
[20] Zhou G., Xiong WX., Zhang XG., Ge SJ. (2013). Retrieval of Consolidated Spatial
Memory in the Water Maze Is Correlated with Expression of pCREB and Egr1 in the
Hippocampus of Aged Mice. Dement Geriatr Cogn Disord Extra 2013;3:39–47. DOI:
10.1159/000348349
[21] Tsuda M et al., (2002). Involvement of an Upstream Stimulatory Factor as Well as
cAMP-responsive Element-binding Protein in the Activation of Brain-derived
Neurotrophic Factor Gene Promoter I*. JBC Papers in Press, July 11, 2002, DOI
10.1074/jbc.M204784200
[22] Palacios D., Summerbell D., Rigby PWJ and Boyes J. (2010). Interplay between DNA
Methylation and Transcription Factor Availability: Implications for Developmental
Activation of the Mouse Myogenin Gene. MOLECULAR AND CELLULAR
BIOLOGY, Aug. 2010, p. 3805–3815 Vol. 30, No. 15 0270-7306/10/$12.00
doi:10.1128/MCB.00050-10
[23] Saunderson EA et al., (2016). Stress-induced gene expression and behavior are
controlled by DNA methylation and methyl donor availability in the dentate gyrus.
4830–4835 | PNAS | April 26, 2016 | vol. 113 | no. 17
24
www.pnas.org/cgi/doi/10.1073/pnas.1524857113
[24] Sherrin T et al., (2010). Hippocampal c-Jun-N-Terminal Kinases Serve as Negative
Regulators of Associative Learning. The Journal of Neuroscience, October 6, 2010.
30(40):13348 –13361
[25] Heurteaux C., Messier C., Destrade and Lazdunski M., (1992). Memory processing and
apamin induce immediate early gene expression in mouse brain. Molecular Brain
Research, 3 (1993) 17-22
[26] Miller CA and Sweatt JD. (2007). Covalent Modification of DNA Regulates Memory
Formation. Neuron 53, 857–869, March 15, 2007 a2007 Elsevier Inc. DOI
10.1016/j.neuron.2007.02.022
[27] Zenk F et al., (2017). Germ line–inherited H3K27me3 restricts enhancer function during
maternal-to-zygotic transition. Science 357, 212 –216 (20 17).
http://science.sciencemag.org/
[28] Anway MD. (2006). Endocrine Disruptor Vinclozolin Induced Epigenetic
Transgenerational Adult-Onset Disease. Endocrinology 147(12):5515–5523. doi:
10.1210/en.2006-0640
[29] Chen Q et al., (2016). Sperm tsRNAs contribute to intergenerational inheritance of an
acquired metabolic disorder. SCIENCE sciencemag.org 22 JANUARY 2016 • VOL 351
ISSUE 6271. http://science.sciencemag.org/
[30] Fischer C et al., (2004). Activating Transcription Factor 4 Is Required for the
Differentiation of the Lamina Propria Layer of the Vas Deferens1. BIOLOGY OF
REPRODUCTION 70, 371–378 (2004). DOI 10.1095/biolreprod.103.021600
[31] Hajkova P et al., (2002). Epigenetic reprogramming in mouse primordial germ cells.
Mechanisms of Development 117 (2002) 15–23. PII: S0925-4773(02)00181-8
[32] Huypens P et al., (2016). Epigenetic germline inheritance of diet-induced obesity and
insulin resistance. Nature Genetics VOLUME 48 | NUMBER 5 | MAY 2016.
doi:10.1038/ng.3527
[33] Chen Z et al., (2017). Impaired learning and memory in rats induced by a high-fat diet:
Involvement with the imbalance of nesfatin-1 abundance and copine 6 expression.
Journal of Neuroendocrinology. 2017; 29:1–12. DOI: 10.1111/jne.12462 |
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