基於PEG的益生元對痤瘡痤瘡桿菌的表皮葡萄球菌發酵和電的研究

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姓名

裘納(Narangerel Choimbol) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

基於PEG的益生元對痤瘡痤瘡桿菌的表皮葡萄球菌發酵和電的研究
(Investigation of fermentation and electricity of Staphylococcus epidermidis using PEG-based prebiotic against Cutibacterium acnes)

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摘要(中)

在人類生活中存在許多微生物，包括共生和致病微生物。人體皮膚的表面有痤瘡丙酸桿菌和表皮葡萄球菌。痤瘡丙酸桿菌和表皮葡萄球菌都是革蘭氏陽性細菌：其中痤瘡丙酸桿菌是厭氧菌，而表皮葡萄球菌在有氧條件下生長最好。痤瘡丙酸桿菌在痤瘡中發揮積極作用，進而在生命中的某些時刻會影響85-100％的人口。雖然尋常痤瘡不會危及生命，但嚴重的痤瘡會嚴重影響患者的心理狀態，從而減少他們對社交活動的參與。皮膚導電性是皮膚電導率的測量。目前發現一些人體皮膚和腸道細菌可以產生電力。因此，我們假設人類皮膚細菌影響皮膚導電性。在該研究中，我們使用2％聚乙二醇10（PEG-10）來誘導表皮葡萄球菌的發酵並產生電壓。我們的研究結果表明皮膚細菌可以產生電能。具有表皮葡萄球菌的2％PEG-10在ICR小鼠中引發皮膚導電性的電子。電子是由表皮葡萄球菌產生的，具有2％PEG-10發酵，其可以抑制痤瘡丙酸桿菌的生長。

摘要(英)

There are many microbiotas including symbiotic and pathogenic microorganisms in humans. The surface of the human skin habits both Cutibacterium acnes (C. acnes) and on the face and Staphylococcus epidermidis (S. epidermidis). Both C. acne and S. epidermidis are gram-positive bacteria: C. acnes are anaerobes whereas S. epidermidis grows best in aerobic condition. C. acnes plays an active role in acne vulgaris, affecting 85-100% of the population at some point in their lives. Although acne vulgaris is not life-threatening, severe acne can greatly burden a patient’s psychological status, and thereby reduce their participation in social activities. Skin conductance is the measurement of the electrical conductivity of the skin. Some human skin and gut bacteria can produce electricity. We thus hypothesize that human skin bacteria affect skin conductance. In this study, we used 2% polyethylene glycol 10 (PEG-10) to induce fermentation of S. epidermidis and generated a voltage. Our results suggest that skin bacteria can produce electricity. 2% PEG-10 with S. epidermidis provoked the electricity in the skin conductance in ICR mice. The electron was produced from S. epidermidis with 2% PEG-10 fermentation which can inhibit growth of the C. acnes.

關鍵字(中)

★ 寻常痤疮
★ 痤疮丙酸杆菌（Cutibacterium acnes）
★ 电
★ 发酵
★ PEG-10（聚乙二醇10）
★ 表皮葡萄球菌（表皮葡萄球菌）

關鍵字(英)

★ Acne vulgaris
★ electricity
★ fermentation
★ PEG-10 (polyethylene glycol 10)
★ S. epidermidis (Staphylococcus epidermidis)
★ C. acnes (Cutibacterium acnes)

論文目次

Abstract ..................... III
Acknowledgments .....IV
List of Figures ..........VII
List of Abbreviations ................................................ VIII
1.1 The skin microbiome ........................................... 1
1.2 Acne vulgaris ..... 1
1.3 S. epidermidis against C. acnes ................................ 3
1.4 Fermentation of bacteria .......................................... 4
1.5 Bacterium .............. 7
1.5.1 Staphylococcus epidermidis ............................... 7
1.5.2 Cutibacterium acnes (Propionibacterium acnes) ................................................. 8
1.6 How bacterial can produce electricity? ..................... 9
1.7 Polyethylene glycol ............................................... 10
1.8 Skin patch ............ 11
1.9 Multimeter detection .............................................. 12
2.1 Materials .............. 13
2.2 Animals ............... 14
2.3 Methods ............... 14
2.3.1 Bacterial Culture .............................................. 14
2.3.2 O. D measurement ........................................... 15
2.4 Medium preparation ............................................... 15
2.4.1 TSB medium preparation ................................. 15
2.4.2 TSB with Phenol Red medium preparation ...... 15
2.4.3 RCM selective agar preparation ....................... 16
2.5 Prolong voltage detection from S. epi with 2% PEG-10 fermentation. ..................... 16
2.6 Determine minimum bactericidal concentration of 2% PEG-10 ........................... 16
2.7 In vivo experiment ................................................. 17
2.7.1 Voltage measurement on mouse back skin treated with S. epidermidis.............. 17
2.7.2 Voltage measurement on mouse back skin treated with PEG-10........................ 18
Chapter 3: Results ...... 19
3.1 Staphylococcus Epidermidis voltage measurement on mice dorsal back skin ........... 19
3.2 Staphylococcus Epidermidis + medium voltage measurement on mice dorsal back skin.................................. 20
3.3 2% PEG-10 + S. epidermidis and 2% PEG-10 + S. epidermidis voltage measurement
on mice dorsal back skin .............................................. 21
3.4 S. epidermidis + medium and S. epidermidis + 2% PEG-10 voltage measurement on
mice dorsal back skin 22
3.5 Prolong voltage detection from S. epi with 2% PEG-10 fermentation. ..................... 23
3.6 Reduction of C. acnes colonization and inflammation in vitro by PEG-10 fermentation
of S. epidermidis ........ 24
3.7 Determine minimum bactericidal concentration of 2% PEG-10 ............................... 25
Discussion and Conclusion .......................................... 26
Reference .................. 28
APPENDIXES .......... 31

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指導教授

黃俊銘(Chun-Ming Huang)

審核日期

2019-7-12

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