Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

NCU Institutional Repository > 理學院 > 光電科學研究所 > 期刊論文 > Item 987654321/28058

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/28058

题名:	Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
作者:	Huang,JC;Chang,YF;Chen,KH;Su,LC;Lee,CW;Chen,CC;Chen,YMA;Chou,C
贡献者:	光電科學研究所
关键词:	LINKED-IMMUNOSORBENT-ASSAY;REVERSE-TRANSCRIPTASE PCR;MONOCLONAL-ANTIBODY;SENSITIVE DETECTION;STOOL SPECIMENS;SPIKE PROTEIN;THROAT WASH;ANTIGEN;AMPLIFICATION;NANOPARTICLES
日期:	2009
上传时间:	2010-06-29 19:41:03 (UTC+8)
出版者:	中央大學
摘要:	In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10(4)-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (similar to 1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection. (C) 2009 Elsevier B.V. All rights reserved.
關聯:	BIOSENSORS & BIOELECTRONICS
显示于类别:	[光電科學研究所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	1151	检视/开启

在NCUIR中所有的数据项都受到原著作权保护.

社群 sharing

数据加载中.....