摘 要 生物科技已經成為全球各先進國家的明星產業之一，而不論是生物體的組織液或是微生物的詴片等，為了保持其生物的活性，都必頇保存在超低溫的液態氮環境中。國內現有的液態氮超低溫 ( -196℃) 儲存設備，例如Taylor-Wharton系統之傳統開蓋式的液態氮儲存槽以及機械式儲存槽 Cryo -Cell系統 (Cryo-Cell International, Inc. USA)、BioArchive系統(Thermogenesis Corp. USA)…等。 本篇論文主要的研究是開發新式液態氮超低溫 ( -196℃) 儲存設備，回顧目前業界的產品，保留其操作與儲存的優點並改善其缺點，進而設計出一個更適合存放生物詴片的超低溫 ( -196℃) 儲存設備。 研究過程中包含硬體與軟體的設計，硬體方面：除了新型第一代儲存槽電腦3D模型的修改、實體加工製作以及實體設備在超低溫液態氮環境下實際使用測詴，另外也持續開發設計新型第二代儲存槽，並且在機構設計初步規劃完成後，使用有限元素法，靜態分析其結構強度，瞭解應力集中處是否會造成結構破壞；軟體方面：撰寫儲存槽人機操作介面管理系統，以及有關儲存槽安全性的感測器即時監控通報系統程式。 研究的成果：實際測詴與分析結果顯示此種儲存系統設計是可行的。儲存槽可利用最簡單的機構設計，使詴片做各別單一存取，可避免影響到其它詴片的生物活性；並且配合儲存槽人機操作系統與即時監控系統使儲存槽系統能達到完善的人機介面整合。 ABSTRACT The progress of biotechnology not merely advents the human welfare, but reveals great economic potential. With the development of technology and the attraction of business profits, the biotechnology is destined to become a nouveau high-tech industry where the whole world will pour into enormous efforts. This study aims to develop a container with liquid nitrogen at -196°C as a more suitable storage for biological samples, by extracting advantage and eliminating limitation from currently-adopted containers. At present, there are the traditional manual lid-opening container by Taylor-Wharton, the mechanical container by Cryo-Cell (Cryo-Cell International Inc., USA), the one by BioArchive (Thermo genesis Corp., USA) and others in Taiwan. The study procedure include hardware and software design, hardware: In addition to the 3D computer model’s changes, entities processed and equipment’s actual test of using of the first-generation storage system, while also continue to develop the design of the second-generation storage system. After the completion of the mechanical structure design, we calculate the structural strength with the finite-element analysis in order to understand whether the stress concentration exceeds the material strength and cause fracture of structure. Software: TO develop an operation and control system with human-machine interface and a sensor’s real-time surveillance system for storage’s security. Research results: the actual test of using and analysis results show that the design of such storage system is viable. With the simplest design, the storage system can just access one single biospecimen for each time and avoid to effect the biological activities of other samples. Finally, to cooperate the operation and control with human-machine interface system and real-time surveillance system.
