金奈米粒子合成及表面化學改質與其應用於DNA分子雜交動力學之探討; Au Nanoparticle Synthesis and Surface Chemical Modification and Its Application for DNA Hybridization Kinetics Study

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Title:	金奈米粒子合成及表面化學改質與其應用於DNA分子雜交動力學之探討;Au Nanoparticle Synthesis and Surface Chemical Modification and Its Application for DNA Hybridization Kinetics Study
Authors:	許景翔;Jing-Hsiang Hsu
Contributors:	化學工程與材料工程研究所
Keywords:	去氧核醣核酸;表面化學改質;金奈米粒子;生物晶片;biochip;surface chemical modification;DNA;Au nanoparticle
Date:	2003-07-02
Issue Date:	2009-09-21 12:21:48 (UTC+8)
Publisher:	國立中央大學圖書館
Abstract:	本研究是利用表面化學改質的方法，在金奈米粒子(Au nanoparticle)表面形成一自我聚集單層膜(self-assembly monolayer; SAM)，探討具有不同官能基之自我聚集單層膜改質對金奈米粒子分散或聚集的影響，並可經由UV/Vis spectrophotometer中之紅移(red shift)現象來量測金奈米粒子聚集之行為。我們也藉由恆溫滴定微卡計(Isothermal Titration Calorimetry; ITC)測量出具有不同官能基單層膜之金奈米粒子的稀釋熱(dilution heat)，來表示金奈米粒子在溶液相中之分散行為。利用表面化學改質的方法與技術，可將不須經過硫修飾的單股DNA接在金奈米粒子上來當做探針(probe)，再與另一互補單股亦接在金奈米粒子上標的(target)DNA進行雜交(hybridization)，我們並改變不同的雜交環境：鹽濃度、雜交溫度及DNA序列中有錯誤鹼基配對等變因來了解其固定化於金奈米粒子的DNA分子雜交特性。本實驗之結果將有助於生物晶片及生物奈米科技之發展。 This study utilized the surface chemical modification methods to form a self-assembly monolayer (SAM) on the surface of Au nanoparticles, and discussed the aggregation or dispersion of the different functional groups SAM modified Au nanoparticles, and via the phenomena of red-shift to determine the behavior of Au nanoparticles aggregation by UV/Vis spectrophotometer. We also determined the dilution heat of different functional group SAM modified Au nanoparticles by isothermal titration calorimetry (ITC) to explain the dispersion mechanism and the behavior of Au nanoparticles in the aqueous phase. Using surface chemical modification methods and techniques, we can immobilize the unmodified single strand DNA on the Au nanoparticles as a probe, and hybridized with the complementary single strand DNA that immobilized on Au nanoparticles as a target. This study, therein investigated the DNA hybridization properties by UV/Vis spectrophotometer. The results of this investigation, hybridization properties were carried out by changing different conditions：salt concentration, hybridization temperature and number of mismatching bases in sequences of target DNA. This kind of experiment can supply the information to develop the biochip and bionanotechnology.
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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