由於老齡化社會趨勢的增加，於自我照護的階段需要有一個工具，可以快速、方便、準確地檢測出疾病的症狀。而生物傳感器即可做為低成本、高效率的檢測裝置應用在我們的日常生活中。本研究將進一步的利用材料科學，化學科學和電子科學，對於阻抗式及壓電式感測技術進行研究及討論。論文中針對不同換能器的研究，以辨識層，換能器和感測電路三個部分來共同建構生物感測系統。其中根據不同的檢測標的，利用一般表面化學處理程序，將專一性的辨識層有效建構在感測器表面。並因為感測器與辨識層的結合，已成功得到阻抗式感測器對於去氧核糖核酸，蛋白質和動物細胞進行感測及應用。其各感測標的物是透過辨識層的專一性捕捉，及換能器的電子信號轉換而完成特定的感測分析。而另一個部分是利用鋯鈦酸鉛壓電元件，做為新型微重量感測器的研究，主要藉由共振頻率的變化來對於生物分子的重量、大小進行感測。這兩種(阻抗式和壓電式)傳感器的研究在本論文中，皆展現出以微製程技術製備感測晶片，以及訊號讀取電路的系統整合。隨後，本論文也對於感測器實際應用上的考量要素，以不同實驗案例，對於阻抗式及壓電式生物感測器在生物醫學及商品化進行評估及討論。As the potential threat of an aging society increases, there is great need for a tool that can quickly, conveniently, and accurately detect the symptom of any disease at the self-care stage. Biosensors can essentially serve as a low-cost and highly efficient device for this purpose in addition to other day-to-day applications. This study discusses advances in impedimetric and piezoelectric sensor technology, which draw on the disciplines of materials, chemistry, and electronics. This study shows that a biosensor with a difference transducer consists of three components, a reorganization layer, a transducer, and an output circuit system. According to different detection targets, the reorganization layer of this study follows common immobilization procedures for efficacious attachment on the transducer surface. Based on different immobilization procedures, this study successfully uses the impedance sensor applied in DNA, protein, and animal cells. Then, a “specific reorganization layer” recognizes a specific analyte to show that the electrical signal utilizes a converted impedance sensor. The other part of the study develops a lead zironate titanate (PZT) chip as a novel sensitive gravimetric biosensor by reducing size and using the resonance feature in biomolecule detection. Two types of transducers in this article, impedimetric and piezoelectric, provide the microfabrication technique of sensing chip and readout circuit formation. Subsequently this article discusses a few practical factors in several experiments as different case studies affecting biomedical application and commercialization of impedimetric and piezoelectric biosensors.
