雙離子性材料為一良好的抗蛋白吸附的材料，為了將雙離子性材料以物理披覆方式附著於疏水材質表面，我們必須將合成同時具有雙離子性片段和疏水性片段的高分子。由於將雙離子性單體與疏水性單體進行共聚合十分不易，我們採二甲基乙二胺Dimethylenediamine （DMEA）與高分子Poly (styrene-co-maleic anhydride) (SMA) 或Poly (Maleic anhydride alt 1-octadecene) (MAO)反應的方式合成同時具有雙離子性片段和疏水性片段的高分子SMA-DMEA及MAO-DMEA。 研究目的在於比較這兩種高分子披覆在PVDF超過濾膜上以及烷類自組裝膜上的抗蛋白吸附特性。我們以吸附平衡後液體中蛋白的減少量，來衡量高分子披覆前後PVDF薄膜對牛血清蛋白Bovine Serum Albumin (BSA)與溶菌酶Lysozyme (LYZ)的吸附量；以SPR測量高分子披覆前後，烷類自組裝膜對BSA與溶LYZ的吸附量；又以ELISA與SPR測量高分子披覆前後，fibrinogen在烷類自組裝膜上的吸附程度。ELISA與SPR測量的結果，顯示SMA-DMEA與MAO-DMEA的披覆均可有效降低fibrinogen在烷類自組裝膜上的吸附。實驗結果也顯示，MAO-DMEA高分子在PVDF膜上有較SMA-DMEA高分子更高的披覆量，而無論是MAO-DMEA或SMA-DMEA高分子的批覆，均顯著的降低BSA吸附量至1µg/cm2。而在LYZ吸附實驗中，顯示SMA-DMEA高分子的批覆可有效阻止LYZ的吸附，但MAO-DMEA的披覆對LYZ的抗吸附能力卻不足。SPR的測量顯示MAO-DMEA披覆於烷類自組裝膜上，可大幅降低LYZ的吸附能力至40 ng/cm2，這結果顯示MAO-DMEA高分子披覆於PVDF膜上，之所以無法有效阻止LYZ的吸附，可能是由於MAO的分子量太大(MW 40K)，無法有效披覆於PVDF膜內的小孔，因此雖然可以阻擋體積大的BSA，卻無法阻止體積小的LYZ。 我們也進行披覆穩定性的實驗，測試經高分子披覆的PVDF膜，在以去離子水清洗1，5，10次後，對BSA與LYZ的抗吸附能力。結果顯示MAO-DMEA附著於PVDF膜的力量較強，披覆穩定性較佳，因此我們認為，分子量較小的MAO-DMEA高分子，應是良好的抗生物沾黏的披覆材料。 Zwitterionic materials have good property of the anti-adhesion of the protein. In order to take the zwitterionic material coated on hydrophobic surface by physical adsorption, we have to synthesize the polymer both have zwitterionic groups and hydrophobic segments. Because it is difficult to take the zwitterionic groups polymerize with the hydrophobic monomer, we choose the Dimethylenediamine (DMEA) react with the Poly (styrene-co-maleic anhydride) (SMA) or Poly (Maleic anhydride alt 1-octadecene) (MAO) to synthesize a SMA-DMEA and MAO-DMEA polymers both have zwitterionic groups and hydrophobic segments. In this study, we compared the protein adsoprion difference on coated polymer PVDF membrane and coated polymer chip. We used the decreased amount of the protein in solution, to measure the Bovine serum albumin (BSA) and Lysozyme (LYZ) adsorption of the coated polymer PVDF membrane; used the ELISA and SPR to measure the fibrinogen adsorption of the coated undecane-1-thiol chip. It is revealed that coated SMA-DMEA and MAO-DMEA membrane can reduce fibrinogen adosption effectively from the SPR and ELISA’s results. The results also showed the MAO-DMEA polymer has higher coating amount than the SMA-DMEA polymer. Not only MAO-DMEA can reduce the BSA adsopriton to 1µg/cm2, but also SMA-DMEA. In LYZ adsoption experiment, SMA-DMEA polymer can reduce LYZ adsorption effectively, but MAO-DMEA has no good anti-adhesion to LYZ. The SPR’s result showed the coated MAO-DMEA chip can significantly reduce the LYZ adsorption to 40 ng/cm2, from this result we cocluded the MAO-DMEA can’t anti-adhesion to LYZ on membrane because of the MAO-DMEA’ molecular weight is too large(MW 50K), it can’t coat on the small hole in PVDF membrane. We also do coating stability experiment the result revealed the MAO-DMEA polymer has higher coating property on PVDF membrane than SMA-DMEA polymer. As a result, we think the smaller molecular weight of the MAO-DMEA maybe is excellent antifouling material.
