本研究利用拋棄式碳電極作為量測工具、交流阻抗法作為檢測分析方法，對血紅素(Hb)與糖化血紅素(HbA1c)進行檢測。拋棄式碳電極以網印方式製成，製作出的電極選用具有較高的相對阻抗差進行實驗量測。由於檢測目標物血紅素與糖化血紅素電特性的不同，在溶液中會產生不同數量的帶電粒子。利用交流阻抗法量測將會由等效電路中元件的改變得到穩定顯著的阻抗差異。藉由此原理可以得知Hb、 HbA1c最大檢測靈敏度範圍與靈敏度。樣本溶血物(hemolystates)在HbA1c不同含量下時，同樣利用交流阻抗法也可得到HbA1c含量不同所相應的阻抗差關係。並藉由交流阻抗法量測原理建立自製阻抗量測平台電路，利用微控制器負責資料傳輸與控制，運用MATLAB進行資料收集與處理。自製電路針對HbA1c量測結果與現行阻抗儀有相近的趨勢，擁有可以彌補現行儀器不易攜帶、昂貴…等問題，搭配拋棄式碳電極可以達成簡易、快速、成本低、攜帶方便的糖化血紅素檢測系統。 This research focused on hemoglobin (Hb) and glycated hemoglobin (HbA1c) detection using disposable carbon electrodes and AC impedance analysis. The disposable carbon electrodes were fabricated by screen-printing technology. Among the three fabricated electrode configurations, the one that led to the largest relative impedance difference was used in the measurement. The natures of Hb and HbA1c are different. They have diffreent amount of charged particles in the solution. By AC impedance analysis, we can obtain stable and significant change in the solution resistance in the equivalent circuit model. By this way we knew the concentration range of Hb and HbA1c corresponding to the maximum detection sensitivity. A self-made circuit was used in the impedance measutement. A microcontroller was responsible for measurement control and data transmission. Measurement data were saved and processed with MATLAB. The HbA1c measurement results were similar with the self-made citcuit and with the commercail impedance analyser, which is expensive and not portable. The self-made citcuit and disposable carbon electrodes have made the measurement easy, fast, inexpensive, and portable.
