Human serum albumin (HSA) is an important biomarker for diagnosing nephropathy. Traditional microalbumin detection is easy affected by the intake of certain medicines, such as aspirin, corticosteroids and antibiotics. In this research, the surface plasmon resonance (SPR) biosensor technique was used to detect HSA. The anthraquinone dye Cibacron Blue F3G-A (CB) is used as a receptor which has a specific affinity for HSA. The purpose of this study is establishing the optimum conditions for SPR to detect HSA. The results exhibited that the sensor chip has high affinity as well as high capacity at pH 4 with 0.05 M sodium chloride. In addition, linear response appeared in the range of 0.01-0.1 mg/ml HSA and the detecting limit was 4 mu g/ml. This methodology may prove to be useful in clinical diagnosis and may serve as an assay for HSA binding ability. (C) 2011 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
