Using isothermal titration calorimetry to real-time monitor the heat of metabolism: A case study using PC12 cells and A beta(1-40)

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Title:	Using isothermal titration calorimetry to real-time monitor the heat of metabolism: A case study using PC12 cells and A beta(1-40)
Authors:	Wang,SSS;Lin,MS;Chen,SL;Chang,Y;Ruaan,RC;Chen,WY
Contributors:	化學工程與材料工程學系
Keywords:	AMYLOID BETA-PROTEIN;ALZHEIMERS-DISEASE;CASCADE HYPOTHESIS;MAMMALIAN-CELLS;PEPTIDE;CHOLESTEROL;FLUX;NEUROTOXICITY;PRECURSOR;MEMBRANES
Date:	2011
Issue Date:	2012-03-27 16:28:01 (UTC+8)
Publisher:	國立中央大學
Abstract:	Isothermal titration calorimetry (ITC) is one of the most powerful means for direct determination of thermodynamic information associated with most physiochemical and biological processes. The deposition and aggregation of beta-amyloid (A beta) on cell membranes was considered as one of the primary factors in having Alzheimer's disease (AD). Recently, a growing body of evidence has suggested that plasma membrane could accelerate the accumulation of A beta on the plasma membranes. However, the mechanism of AD is still a controversial issue. This study provided a biothermodynamic approach to real-time monitor the heat of metabolism involved in the co-incubation of PC12 cells and A beta(1-40) by ITC. The effects of A beta conformation and concentration of oligomeric A beta on cytotoxicity were successfully distinguished by ITC. This approach with rapid and direct measurement may provide not only real-time information for the effects of A beta species on living cells but also a platform for the screening of drug candidates for AD. (C) 2010 Elsevier B.V. All rights reserved.
Relation:	COLLOIDS AND SURFACES B-BIOINTERFACES
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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