Myogenic regulatory factors regulate M-cadherin expression by targeting its proximal promoter elements

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题名:	Myogenic regulatory factors regulate M-cadherin expression by targeting its proximal promoter elements
作者:	Hsiao,SP;Chen,SL
贡献者:	生命科學系
关键词:	SKELETAL-MUSCLE CELLS;HELIX-LOOP-HELIX;BOX TRANSCRIPTION FACTORS;MOLECULE M-CADHERIN;N-CADHERIN;ADHESION MOLECULE;TERMINAL DIFFERENTIATION;MYOD;GENE;MYOBLASTS
日期:	2010
上传时间:	2012-03-27 18:09:35 (UTC+8)
出版者:	國立中央大學
摘要:	M- and N-cadherin are members of the Ca(2+)-dependent cell cell adhesion molecule family. M-cadherin is expressed predominantly in developing skeletal muscles and has been implicated in terminal myogenic differentiation, particularly in myoblast fusion. N-cadherin-mediated cell cell adhesion also plays an important role in skeletal myogenesis. In the present study, we found that both genes were differentially expressed in C2C12 and So18 myoblasts during myogenic differentiation and that the expression of M-cadherin was preferentially enhanced in slow-twitch muscle. Interestingly, most MRFs (myogenic regulatory factors) significantly activated the. promoter of M-cadherin, but not that of N-cadherin. In line with this, overexpression of MyoD in C3H10T1/2 fibroblasts strongly induced endogenous M-cadherin expression. Promoter analysis in silico and in vitro identified an E-box (from 2 to -1-4) abutting the transcription initiation site within the M-cadherin promoter that is bound and differentially activated by different MRFs. The activation of the M-cadherin promoter by MRFs was also modulated by Bhlhe40 (basic helix loop helix family member e40). Finally, chromatin immunoprecipitation proved that MyoD as well as myogenin binds to the M-cadherin promoter in vivo. Taken together, these observations identify a molecular mechanism by which MRFs regulate M-cadherin expression directly to ensure the terminal di fferentiation of myoblasts.
關聯:	BIOCHEMICAL JOURNAL
显示于类别:	[Department of Life Science] journal & Dissertation

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