本文介紹一些光學顯微鏡的觀念與原理,使用軟體Code V來模擬設計光學顯微鏡系統中鏡頭的部分,並且說明了一些關於系統組合時該注意的細節。文中分開設計物鏡:10X/0.25與20X/0.45;目鏡:10X/22,物目鏡除了可以個別使用外,還能將兩者組合成100倍與200倍的系統,所有的成像品質判斷標準皆以Strehl ratio大於0.8來做為其評價。The principal of how to design a microscope is introduced in this dissertation. There three designs in this thesis: two objectives and an eyepiece, and there specifications are 10X/0.25, 20X/0.45 and 10X/22, respectively. By combining the objectives and the eyepiece, we can make 100X and 200X microscope systems.Code V is used to help us simulate the system, and many details of aberrations and the image quality of the microscope system can be observed in the software. In addition, Strehl ratio of an optical system is a diffraction limited system while the value of a system is higher than 0.8. Therefore, the Strehl ratio in the program is considered to examine the image quality of our microscope system.