數位全像術係將傳統全像術之紀錄介質以電子式的感光元件來取代,並能以數值計算的方式來重建物體的波前。數位全像術之優勢在於能將光學平行處理的特點和電子元件結合,達到更為廣泛的應用。 因此,本論文主要探討將數位全像顯微鏡架構微小化的方法。我們利用體積全像光學元件的特性,取代傳統分光鏡,以達到將數位全像顯微鏡的尺寸微小化。另外,當物體非常靠近感光元件時,我們提供了許多方法來取得高解晰度的還原影像,像是:球面波照射光、球面波參考光、線性內插法。最後,並以模擬和實驗來展示此微型化的結果。Digital holography is a technique replacing the conventional holographic recording media by digital sensors. It utilizes numerical methods to reconstruct the wave front of an object. Digital holography not only takes the advantage of the optical characteristic of parallel processing but also combining this advantage with electronic devices. This study devotes to minimize the size of digital holographic microscope. It is realized by using a volume holographic optical element to replace the conventional beam splitter. Multi calculation methods including spherical wave illumination, spherical wave reference and interpolation technique are proposed to achieve high resolution when the objects is very close to the image detector. The ideas have been demonstrated by both simulation and experiment.