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| 題名: | [學研計畫] 人類誘導型多能性幹細胞與人類間葉幹細胞利用接有奈米片段之生醫材料用於幹細胞生產、純化、培養與分化( I );Biomaterials Having Nano-Segments for Generation, Purification, Cultivation, and Differentiation of Human Induced Pluripotent Stem Cells (Ipscs) and Human Mesenchymal Stem Cells (Hmscs)( I ) |
| 作者: | 樋口亞紺;凌慶東;張雍;武國璋 |
| 貢獻者: | 國立中央大學化學工程與材料工程學系 |
| 關鍵詞: | 醫學工程;生物技術(醫) |
| 日期: | 2012-12-01 |
| 上傳時間: | 2014-03-17 11:29:24 (UTC+8) |
| 出版者: | 行政院國家科學委員會 |
| 摘要: | 研究期間：10108~10207;Millions of people suffer from loss and damage of organs and tissue every year because of accidents, birth defects, and diseases. Stem cells are an attractive source of cells in regenerative medicine. Although embryonic stem cells (ESCs) are attractive, they face immune rejection after transplantation; furthermore, there are ethical issues regarding use of human embryos. These concerns can be overcome by induced pluripotent stem cells (iPSCs), if iPSCs can be derived safely from patients’ somatic cells. Their pluripotent nature opens avenues for potential stem cell-based regenerative therapies as well as mesenchymal stem cells (MSCs) such as adipose-derived stem cells (ADSCs) and amniotic fluid stem cells (AFSCs). However, there are several barriers to the use of iPSCs in clinical applications. These include the necessity of using viral vectors to transform the cells, as well as the extremely low probability of generation of iPSCs in some cases. The drawbacks of clinical usage of iPSCs is also that iPSCs are currently cultured on mouse embryonic fibroblasts (MEFs) as a feeder layer. It is desirable to culture iPSCs in xeno-free condition for clinical usages. MSCs are also a promising cell source in regenerative medicine as well as iPSCs. However, there are limited cell fates associated with pluripotency in these cells. It is difficult to obtain large quantity of MSCs and to maintain the pluripotency of MSCs for a long time in culture. It will be promising if we can purify and culture MSCs from tissue digested solution by a simple and easy method such as a specific filtration method using membranes having specific nanosegments or culture method on biomaterials having specific nanosegments. The goal of this project is (a) Development and preparation of biomaterials having specific nanosegments for long-term culture of human iPSCs and hMSCs with pluripotency, (b) Development and preparation of biomaterials having specific nanosegments for differentiation of iPSCs and MSCs into specific and desired lineages (e.g., neural cells secreting dopamin for Parkinson disease treatment or beta cells secreting insulin for diabate treatment), (c) Preparation of human iPSCs by gene (or miRNA and pluripotent proteins) induction using the nanocarriers, (d) Development of purification and isolation of MSCs by membrane filtration method through membranes having nanosegments and by culture on biomaterials having nanosegments, and (e) Characterization and differentiation of MSCs and iPSCs. We will develop an easy method for isolation of MSCs from tissue digested solution by membrane filtration method using membranes having specific nanosegments, and by culture method on biomaterials having specific nanosegments. We will further develop biomaterials having nanosegments, which guide pluripotency and differentiation fate of iPSCs and MSCs. We will also develop non-viral and nanosize particles that have fluorescent probes and/or magnetic nanoparticles as carriers of pluripotent genes, miRNA, or pluripotent proteins to generate iPSCs and will select iPSCs using magnetic force or fluorescence intensity using fluorescence microscopy or FACS. This will enable us to generate iPSCs with high efficiency, where the iPSCs developed in this project are highly reproducible and safe. |
| 關聯: | 財團法人國家實驗研究院科技政策研究與資訊中心 |
| 顯示於類別: | [化學工程與材料工程學系 ] 研究計畫
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