全球糖尿病的病患逐年增加，糖尿病容易引發許多併發症致死率相當高，現今的檢測方式多為侵入式，為了免去病人其痛苦，建立一個非侵入式的檢測平台，透過奈米矽結構結合奈米金粒子，製作一個奈米尺度的化學反應與質譜分析的快速檢測平台，本文以奈米矽結構為基材沉積奈米金粒子，利用奈米矽結構本身的吸附性，吸附葡萄糖進入奈米矽，接觸金產生還原氧化反應，反應完的樣本留在晶片上，使用MALDI-TOF質譜儀將雷射打在晶片上，讓樣本脫附飛梭進入檢測器，以達到反應檢測的效果。 由於要建立的平台為非侵入式，因此以檢測病患的尿液為的目標，在人體的尿液中有相當多的雜質及鹽分，這對於檢測分析而言具有相當大的干擾性，本人參考了氧化酶電極法的催化機制，透過具有高度的專一性的酶，將催化機制應用於奈米矽結構上，本人使用金觸媒取代一般傳統葡萄糖氧化酶作為催化物。 經過實驗發現奈米尺度的金具有良好的催化能力，再加上質譜分析儀的應用提高整個平台的靈敏性，以標準品葡萄糖為檢測對象可以檢測至10-8M，之後將此高靈敏性的平台應用於尿液的檢測上，使用正常人的尿液混合加入10-3M的葡萄糖，進行檢測分析具有良好的訊號強度，此結果證實奈米金的應用有助於分析葡萄糖，並且提高其專一性及靈敏性。; Diabetic patients increases ni the global year by year. The disease is that easy to trigger many complicating disease and have high fatality rate. Recently , detect it has many method , but mostly method is invasive. We want to establish a non-invasive detection platform by nanostructure silicon (nSi) surface combination gold nanoparticle(AuNPs) for less patient pain. This detection platform have nano-scale,chemical reaction and rapid analysis with Mass Spectrometry. In this study, Depositing AuNPs on nSi surface,we call it sd-nSi chip. Use the chip surface properties absorption glucose to inside to oxidation reaction with AuNPs. After the oxidation reaction, employ MALDI-TOF mass spectography laser hit sd-nSi surface let the sample shuttle into a detector to achieve the detection. Because of our method is invasive,so we chose human urine detected. But in human urine have many impurity and salt that for detect is not easy. As a result we use catalytic mechanism on nSi by enzymes and take AuNPs replace GOx. In our experiment,finding that the platform combine AuNPs have high sensitivity for standard glucose even to clinical test still have good sensitivity.
