Glutathione為KB細胞株中對抗亞砷酸鈉毒性之主要物質;Glutathione is a major participant in the defense of KB cells against NaAsO2-elicited toxicity

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题名:	Glutathione為KB細胞株中對抗亞砷酸鈉毒性之主要物質;Glutathione is a major participant in the defense of KB cells against NaAsO2-elicited toxicity
作者:	王怡婷;Yi-Ting Wang
贡献者:	生命科學研究所
关键词:	榖胱甘肽;砷化物;亞砷酸鈉;抗氧化物;氧化壓力;酵素活性;antioxidant;sodium arsenite;arsenic;glutathione;enzyme activity;reactive oxygen species;ROS
日期:	2007-07-03
上传时间:	2009-09-22 10:20:16 (UTC+8)
出版者:	國立中央大學圖書館
摘要:	近年來研究指出，砷化物的毒性作用機制之一為促使細胞內氧化壓力(oxidative stress)的產生。本研究主要目的為探討三價無機砷 (NaAsO2, As(III))是否導致人類口腔癌細胞(KB cells)中超氧化物 (reactive oxygen species, ROS)含量上升以及其對於細胞內中各種抗氧化物質之影響，並藉由專一性抑制劑與RNA 干擾技術分別抑制細胞中抗氧化物質，以瞭解各種抗氧化物在砷化物毒性中所扮演的角色。結果顯示，當細胞經不同濃度(0~40 ?M) 之As(III) 處理24 小時後，細胞內的ROS 有隨著砷化物劑量增加而誘導生成之情形。進一步分析細胞內抗氧化物質後發現超氧歧化酶(SOD)、榖胱甘肽過氧化酶 (GPX)之酵素活性與peroxiredoxins 的基因表現並無受到As(III) 之影響。然而，榖胱甘肽轉移酶(GSTs)和觸酶(catalase)的酵素活性以及 GST?、perxiredoxin 6 的蛋白表現卻在高濃度As(III)處理下反而受到抑制。僅有細胞內榖胱甘肽(GSH)之含量會被As(III) 所大量誘導生成；進一步探討兩種GSH 生合成有關的蛋白glutathione reductase (GR) 以及?-glutamylcysteine synthase (?-GCS)，亦發現其基因與活性表現皆會受到As(III)所誘導。利用抑制劑BSO 抑制細胞內GSH 的合成，使得細胞對砷化物之毒性更加敏感，且在低濃度砷化物處理之下，就會導致細胞內ROS 的顯著累積。因此本實驗推論KB 細胞對抗As(III) 之毒性，主要藉著誘導GSH 合成酶活性增加，進而使細胞內GSH 大量表現以維持細胞氧化還原當量的平衡，並減少ROS 之累積以達到保護細胞之功用。 Arsenic is a common environmental toxicant and one of the plausible mechanisms of arsenic toxicity is oxidative stress. Oxidative stress has also been implicated as a possible etiologic factor for arsenic carcinogenesis. The objective of this research is to study the effect of sodium arsenite (NaAsO2, As(III)) on antioxidant defense activities in a human carcinoma KB cell line. The results showed that the survival of KB cells was decreased as the dose of As(III) increased. Moreover, treatment of the cells with 0-40 ?M As(III) for 24 hours enhanced the formation of cellular reactive oxygen species (ROS). However, these treatments had no effect on the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) as well as on the mRNA expressions of peroxiredoxins. Moreover, the activities of glutathione S-transferases (GSTs), catalase and the protein levels of GST?, catalase and Prx6 were down-regulated in As(III)-treated KB cells. In contrast, the level of glutathione (GSH) represented the sole antioxidant that up-regulated in KB cells by As(III) treatment. The results also showed that glutathione reductase (GR) and ?-glutamylcysteine synthase (?-GCS) appeared to be the major contributing factors to the higher level of GSH observed in As(III)-treated cells. Depletion of GSH sensitized KB cells to As(III) treatment and enhanced ROS formation in cells, while depletions of other antioxidants had no significant effects on As(III)-induced cytotoxicity. These results supported the hypothesis that GSH is a major participant in the defense of KB cells against As(III)-elicited toxicity.
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