於蛋白質體學（proteomics）研究上，建立一多功能光學生物感測平台作為生物分子交互作用分析（biomolecular interaction analysis，BIA）為一重要研究工作。藉由表面電漿共振（surface plasmon resonance，SPR）感測技術來提供生物分子交互作用的動力學（kinetics）資訊，並搭配可提供生物分子的結構資訊之表面強化拉曼散射（surface-enhanced Raman scattering，SERS），可建立一更完善之生物分子辨識平台。利用此兩種感測機制，將可同時觀察生物分子之動態交互作用及其結構變化情形。 SERS技術具有探測單一分子的能力，可與SPR技術一樣利用衰減全反射方式（attenuated total reflection，ATR）來激發表面電漿子（surface plasmons，SPs）來檢測感測器表面上生物分子。然而由於生物分子拉曼訊號相當微弱，須藉由粒子電漿子（particle plasmons，PPs）的操控，來加以放大。論文中，先介紹SPs與PPs之近場電磁強化作用，藉以作為SERS之物理機制之架構，並以A. Otto先生所提的化學強化機制為輔助，以期對電漿子強化拉曼訊號的機制有理論上之依據。在實驗部份，利用射頻混合濺鍍與化學方式奈米銀粒子的備製，來設計操控界面上奈米膜層之金屬粒子大小與分佈情形，並以自製的ATR-SPR光譜儀與微拉曼光譜儀來加以量測分析。最後以copper phthalocyanine（CuPc）及去氧核糖核酸（DNA ）之SPR與SERS光譜之量測訊號加以分析討論，作為與本論文研究動機與目的之驗証。經由這些實驗結果，我們將可建構一藉由電漿子來強化量測訊號之SPR及SERS的生物分子辨識平台，除了提供生物分子交互作用的即時動態反應並且提供生物分子的結構改變之資訊。 To establish a multi-purpose optical biosensing platform for biomolecular interaction analysis (BIA) is a key research work in proteomics. With helps of surface plasmon resonance (SPR) to analyze the kinetics of biomolecular interactions and of surface-enhanced Raman scattering (SERS) to detect the structural change of biomolecules, an advanced biomolecular recognition system with the both techniques can provide more information in a variety of BIA. SERS can be used to identify the molecular structure on sensor surface with the enhancement of electro-magnetic (EM) field through exciting surface plasmons (SPs) based on the attenuated total reflection (ATR) method. However, the Raman signal of biomolecule is still tiny, and hence it is needed to be magnified by other approaches such as manipulating particle plasmons (PPs). In this thesis, the near-field EM enhancement through SPs and PPs excitation is introduced first. The physical and chemical mechanisms of SERS are investigated to provide a scientific basis for understanding the plasmonic enhancement of Raman signal. Using both a radio-frequency co-sputtering method to fabricate Au@SiO2 composite film and a chemical synthesis approach to fabricate Ag nanoparticles, the size and distribution of embedded metal nanoparticles can be controlled on the sensor surface to enhance the near EM field. Finally, the SPR and Raman signal of copper phthalocyanine (CuPc) and deoxyribonucleic acid (DNA ) by using the homemade ATR-SPR and micro-Raman spectroscopes are tested and verified the motivation and intent of this thesis. According to these preliminary achievements, the biomolecular recognition platform utilizing the plasmonic enhanced signal of SPR and SERS, not only provides the real time kinetic information of biomolecular interactions, but also offers the structural information of biomolecules.
