本研究嘗試發展載藥複合電紡纖維。首先我們將不同比例的奈米碳管摻混 入聚乳酸以強化電紡纖維，藉由加入導電的奈米碳管，可以得到細且均一直徑 的奈米纖維，雖然過高奈米碳管的添加可能會因為碳管的聚集降低纖維機械強 度，但我們發現含有0.5 wt%奈米碳管的聚乳酸纖維(0.5 CNT)有著最出色的機 械強度表現。為了調控藥物的釋放行為，我們將不同含量的親水性聚乙二醇摻 混入0.5 CNT 纖維。透過接觸角、FTIR 與XPS 實驗，確認聚乙二醇有確實摻 混入纖維內，雖然PEG 的添加會降低機械強度，但整體而言仍優於純聚乳酸纖 維。且PEG 的加入可有效降低纖維的Tg 而增進纖維延展性。而細胞培養實驗 顯示這些纖維絲均有良好的生物適合性，可有利於細胞貼附生長。最後，我們 將能夠誘導成骨分化的Dexamethasone(Dex)藥物載入纖維中，並利用FTIR 與 XPS 確認藥物有裝載入纖維。其中XPS 的結果顯示奈米碳管的添加可有利於使 Dex 均勻分布於纖維整體。而在藥物釋放曲線中，可以發現聚乙二醇摻混入纖 維後能提高Dex 釋放速率。這些結果顯示，載藥聚乳酸/多壁奈米碳管/聚乙二 醇複合纖維不僅提升了機械強度，且能調控藥物釋放行為，對於骨組織修復與 再生的應用極具潛力。 關鍵字：電紡絲、載藥支架;In this study, composite electrospun fibers were fabricated to develop drug loaded scaffolds. Firstly, different ratios of multi-walled carbon nanotubes (MWCNTs) were incorporated to polylactic acid (PLA) to strengthen electrospun fibers. Compared to thick PLA with random diameters, the use of MWCNTs resulted in uniform and thin fibers due to their good conductivity. Although excess MWCNTs in polymer may aggregate together, fibers with 0.5 wt% MWCNTs (0.5 CNT) performed the best mechanical property. To modulate drug release behavior, different ratios of hydrophilic polyethylene glycol (PEG) were applied to 0.5 CNT. The PEG incorporation was examined by water contact angle, FTIR, and XPS analyses. Although the mechanical strengths of PEG containing fibers decreased with PEG ratios, they were still higher than that of the PLA only fibers. In addition, incorporated PEG reduced Tg of spun fibers and enhanced ductility. The cell culture experiments suggested that fabricated fibers all demonstrated good biocompatibility. Finally, dexamethasone (Dex), an osteogenic inducer, was loaded to PLA/MWCNT/PGE fibers and was confirmed by FTIR and XPS. The XPS results suggested that loaded Dex mainly stayed the surfaces of PLA fibers, whereas even distributed in MWCNTs containing fibers. Drug release experiments demonstrated that released Dex was manipulated by PEG ratios that the more PEG in fibers, the faster Dex released. These properties indicated that our developed drug-loaded PLA/MWCNT/PEG fibers not only promoted mechanical strength, but also regulated drug release behavior, thus should be potential to bone tissue regeneration application. Key words：electrospun、drug-loaded scaffolds
