以鄰苯二酚與金屬離子螯合方式形成抗菌及抗污之表面塗層研究;Development of Metal-Phenolic Networks for Antimicrobial and Antifouling Properties

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題名:	以鄰苯二酚與金屬離子螯合方式形成抗菌及抗污之表面塗層研究;Development of Metal-Phenolic Networks for Antimicrobial and Antifouling Properties
作者:	鄭筱彤;Cheng, Hsiao-Tung
貢獻者:	生醫科學與工程學系
關鍵詞:	抗非特異性吸附;多巴胺;鄰苯二酚;聚乙二醇;金屬離子
日期:	2017-07-26
上傳時間:	2017-10-27 13:10:16 (UTC+8)
出版者:	國立中央大學
摘要:	醫療器材裝置通常伴隨著蛋白質，細胞和細菌等大量非特異性吸附。這些問題最終會在醫療上引起不良的致病性，如血栓的形成以及生物材料相關的感染問題。在本研究中，我們利用酚團結構的親水性高分子與二價或三價鐵離子在鹼性溶液中形成複合物 (metal-phenolic complex network, MPN) ，發展出簡單且不受基板大小及化學組成的表面修飾方法，於各種表面進行改質，進而達到抗生物汙染的特性。此篇採用貽貝啟發的鄰苯二酚衍生物—多巴胺甲基丙烯酰胺 (dopamine methacrylamide, DMA)，並利用隨機聚合反應 (free radical polymerization) 將多巴胺甲基丙烯酰胺 (DMA) 與聚乙二醇甲基丙烯酸酯 (polyethylene glycol methacrylate, PEGMA) 形成聚乙二醇甲基丙烯酸酯-多巴胺甲基丙烯酰胺共聚物 (polyethylene glycol methacrylate-co-dopamine methacrylamide, PEGMA-co-DMA)。因鄰苯二酚的結構與二價及三價鐵離子皆能形成複合物 (MPN) ，將能修飾在多種基材表面之上。首先利用1H NMR與凝膠滲透層析儀確定高分子的結構與分子量。接著使用紫外-可見分光光度法計算PEGMA-co-DMA 與二價及三價鐵間的平衡常數。然後使用水接觸角、X射線光電子能譜及原子力顯微鏡 (atomic force microscopy, AFM) 了解表面修飾後的親水性質、表面元素組成及表面粗糙度。最後藉由表皮葡萄球菌及綠膿桿菌進行細菌貼附，再利用纖維母細胞做細胞貼附測試，比較不同價數的鐵離子與PEGMA-co-DMA修飾的薄膜，是否有不同抗生物汙染程度及殺菌的功效。本研究的最終目的是發展出一種新的修飾方法，可於各種基材表面進行改質進而達到抗生物汙染的功能，並期待應用於醫療器材上，以提升生物相容性與安全性。;Medical devices are often accompanied by large amounts of non-specific adsorption of proteins, cells and bacteria. These problems will eventually cause poor medical pathogenicity, such as the formation of thrombosis and biomaterials associated infection. In this work, we report a facile strategy for preparation of surface-independent and low-fouling coating via coordination of polyphenol with metal ions in aqueous solution. This approach incorporates bio-inspired polymers which containing dopamine methacrylamide (DMA) and poly(ethylene glycol) methacrylate (PEGMA). The product was named polyethylene glycol methacrylate-co-dopamine methacrylamide (PEGMA-co-DMA) for fouling resistance. Herein, metal ions serve as crosslinking agents to react with catechol groups. Film formation was accomplished with the adsorption of the metal-phenolic network (MPN) on various substrates. Here we compared two different charge of metal ions: ferrous ion (Fe2+) and ferric ion (Fe3+). Polymer characterization was carried out with proton nuclear magnetic resonance (1H NMR) spectroscopy and gel permeation chromatography (GPC). The binding constant between iron ions and PEGMA-co-DMA was analyzed by ultraviolet–visible spectroscopy (UV-Vis). The surface hydration, surface elemental composition and the patterns of the modified substrates were confirmed by water contact angle measurements, x-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). For examining the antifouling properties, we immersed the modified substrates into the solutions containing bacteria or cells. Then the adsorbed bacteria or cell was quantified by fluorescence microscopy and cell imaging analysis. This approach provides a deep understanding of a facile way to prepare coating materials biofouling resistance, which can give great inspiration for the design and synthesis of bifunctional coating materials.
顯示於類別:	[生物醫學工程研究所 ] 博碩士論文

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