化學鍍黃金電極之葡萄糖感測器製備及測試

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Title:	化學鍍黃金電極之葡萄糖感測器製備及測試
Authors:	梁供才;Liang, Kung-Tsai
Contributors:	化學工程與材料工程學系
Keywords:	生物感測器;黃金電極;化學鍍;電化學測試;biosensors;gold electrode;electroless plating;electrochemistry test
Date:	2018-06-26
Issue Date:	2018-08-31 11:30:14 (UTC+8)
Publisher:	國立中央大學
Abstract:	現代人因為飲食或作息不正常，造成罹患糖尿病的人逐漸變多，也增加了血糖試紙的需求量。一般黃金電極以物理濺鍍或化學氣相沉積法製成，但此方法設備成本昂貴，本研究以成本低的化學鍍方式成功製作出黃金電極的血糖試紙。利用含有鈀觸媒之油墨以網版印刷的方式，將圖形印刷在PET板子上作為基底，再透過化學鍍製程鍍上鎳、鈀和金層得到最終電極，接著滴覆上酵素層並上蓋絕緣層完成黃金電極的血糖試紙。用製作好的黃金電極進行光電子能譜儀、附著力測試、厚度分析和電化學測試，其中電化學測試主要以循環伏安法與計時安培法進行研究，並探討酵素成份中葡萄糖氧化酶和鐵氰化鉀的組成比例，最後比較碳電極和黃金電極的差異。結果顯示以鍍鎳槽80 ℃下3-5分鐘得到0.3 - 0.5 µm，鍍鈀槽53 ℃下5分鐘得到~0.03µm厚度，鍍金槽80 ℃下8分鐘得到0.04 - 0.06µm為最佳條件的鎳-鈀-金電極，且成功放大製作在38 cm X 33 cm大小的PET基板進行得到最佳黃金血糖試紙。而酵素成份中以一倍葡萄糖氧化酶和兩倍鐵氰化鉀測試結果反應最好。最後透過比較精密度、穩定時間及添加維生素C等干擾物質，化學鍍製成的黃金電極均優於碳電極。 ;Due to irregular diet or routine, more and more people of modern age are suffering from diabetes and increase the demand for blood glucose test strips. Gold electrodes are generally made by physical sputtering or CVD, these methods are expensive. In this study, gold electrode test strips were successfully fabricated by a low-cost electroless plating method. The PET substrate was produced by screen printing method using palladium as the catalyst. After electroless plating process, Ni, Pd and Au were plated to obtain the final electrode. The enzyme layer was dripped and the insulating layer was overlaid to complete the blood glucose test strip. The samples were characterized by X-ray photoelectron spectroscopy, adhesion test, cyclic voltammetry and amperometric methods. The compositions of glucose oxidase and potassium ferricyanide in the enzyme components were compared. The differences between the carbon electrode and the gold electrode were compared. The results show that the optimum condition to deposit the nickel layer was 0.3 to 0.5 μm at 80 °C for 3 to 5 minutes, for palladium layer was about 0.03 μm at 53 °C for 5 minutes, and for gold layer was 0.04 to 0.06 μm at 80 °C for 8 minutes. It is successfully to manufacture on PET substrate with a size of 38 cm X 33 cm to obtain the best gold glucose test strip. The results of the test with single GOD and double potassium ferricyanide showed the best response. Finally, gold electrodes made of electroless plating are superior to carbon electrodes through comparison of precision, stability time, and addition of interfering substances such as ascorbic acid.
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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