| 摘要: | 過去幾十年研究中，彈性模數、表面自由能及兩性離子材料對海洋抗污塗層的發展有重大的影響。本研究期待結合低彈性模數材料聚二甲基矽氧烷（polydimethylsiloxane, PDMS）和兩性離子材料磺基甜菜鹼矽烷（sulfobetaine silane, SBSi）作為一種新的抗海洋生物汙損（anti-marine biofouling）塗層，並以藤壺（Amphibalanus amphitrite）的腺介幼蟲（cyrpids）和石蓴（Ulva lactuca, Ulva fasciata）的游動孢子（zoospores）作為抗海洋生物污損之研究物種。本研究主要進行腺介幼蟲錄影追蹤觀測、腺介幼蟲附著檢測及石蓴孢子附著檢測，其中使用玻璃、Fluorosilane、PDMS、SBSi四種表面。於腺介幼蟲錄影追蹤觀測中，影像顯示它沒有附著的傾向抑或是無法附著在PDMS-SBSi表面上，同時，PDMS-SBSi表面在兩天的腺介幼蟲附著檢測中只有2%的腺介幼蟲附著率；在石蓴孢子附著檢測中，PDMS-SBSi表面上有最小的游動孢子附著數量以及水流沖擊後的92％孢子去除率。由此可見，PDMS和SBSi的組合確實具有對藤壺幼蟲和石蓴孢子的抗污染性，而且，在對抗石蓴孢子時，這個組合更展現了優異的污損釋放特性。基於這些測定，我們將可以進一步探討PDMS-SBSi對藤壺幼蟲的污損釋放，或是深入研究表面的化學性質，使用表面電漿共振儀（SPR）來檢測附著誘導蛋白複合物（SIPC）是否吸附在PDMS-SBSi上。;In the past few decades, elastic modulus, surface free energy and zwitterionic materials have been a key element of the development of marine antifouling coatings. This study combines a low elastic modulus material (polydimethylsiloxane, PDMS) and a zwitterionic material (sulfobetaine silane, SBSi) as a new anti-marine biofouling. Barnacle cypris larva (Amphibalanus amphitrite) and Ulva zoospores (Ulva lactuca, Ulva fasciata) are used as research species against marine biofouling. This study mainly carried out video tracking observation of cyprids (barnacle cypris larva), cyprids settlement assay and Ulva zoospores settlement assay, which used glass, fluorosilane, PDMS and SBSi. In the video tracking, the film showed that it had no tendency to adhere or could not adhere to the surface of PDMS-SBSI. At the same time, only 2% of the cyprids settled on the surface of the PDMS-SBSI for two days. In the settlement assay of Ulva zoospores, the attached number of zoospores is lower on the surface of PDMS-SBSI and the 92% of the attached zoospores removed after water flow impact. The study result indicate that the combination of PDMS and SBSI does have anti-contamination against barnacle cypris larva and Ulva zoospores, also, this combination exhibits superior fouling release characteristics when against Ulva zoospores. Based on these measurements, we will further investigate the stain release of PDMS-SBSI on barnacle cypris larva, or in-depth study of surface chemistry, using surface plasma resonator (SPR) to detect adhesion-induced protein complex (SIPC) adsorption on PDMS-SBSI. |
