針對體表燒燙傷傷口癒合研製具備抗菌及增強細胞生長功能之殼聚醣水凝膠---材料開發與功能性驗證;Design and Fabrication of an Antimicrobial Cell Growth Enhanced Chitosan Hydrogel for Burn Wound Healing --- Material Development and Function Validation

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題名:	針對體表燒燙傷傷口癒合研製具備抗菌及增強細胞生長功能之殼聚醣水凝膠---材料開發與功能性驗證;Design and Fabrication of an Antimicrobial Cell Growth Enhanced Chitosan Hydrogel for Burn Wound Healing --- Material Development and Function Validation
作者:	吳泰里;Wu, Tai-Li
貢獻者:	生醫科學與工程學系
關鍵詞:	銀離子抗菌;水凝膠;傷口癒合;殼聚醣;表皮生長因子;Silver ions;hydrogel;wound healing;Chitosan;EGF
日期:	2020-12-18
上傳時間:	2021-03-18 16:51:23 (UTC+8)
出版者:	國立中央大學
摘要:	燒燙傷的傷口伴隨著形成水泡和腫脹的發生導致極度疼痛，傷口也很容易受到細菌感染，引發敗血症、蜂窩性組織炎…等，需要立即就醫。在這項研究中，我們主要是通過設計和製造含銀離子和表皮生長因子奈米粒子(Chitosan-Sodium Triphosphate encapsulated EGF，CTENPs)的合成殼聚醣水凝膠（Chitosan-PVA including CTENPs and Silver ions Hydrogel，SCPEHG)），設計和製造一種改善燒燙傷創面癒合水凝膠，這是一種有效率的策略，可以同時提供抗菌和加速治癒功能。EGF的殼聚醣奈米粒子是通過使用正負離子作交聯凝聚製成的。透過DLS分析，EGF的奈米顆粒的大小和表面電荷分別為352 ± 16 nm。根據抗菌、細胞毒性和細胞生長檢測效果，確定SCPEHG最佳濃度是24 mM? Ag?^+和60 μg/mL EGF是很有效的。在體外實驗中可發現仍有96%以上的細胞存活率，我們還可以注意到該細胞的生長隨著EGF奈米粒子濃度越高生長越快。我們對SCPEHG進行了釋放測試，EGF水凝膠在PBS中在48小時內釋放為187 ng/mL，發現複合水凝膠能夠提供持續釋放的?" Ag" ?^"+" 和EGF，表明開發的SCPEHG非常適合在富含離子環境下治療燒燙傷。總結，開發出來水凝膠在治療傷口和增強細胞生長方面具有很高優勢。;The wound region of second degree burn involves epidermis and portions of upper dermis and lower dermis. It is formed with blistered and swollen, and is easily infected with microbiomes, result in extremely painful. The clinical syndromes include fever, erythema, edema, suppuration. That require immediate medical treatment to avoid further complications. In this study, we aim to provide an effective strategy for an improved burn wound healing efficiency through design and fabrication of a synthetic chitosan-based hydrogel containing silver ions and epidermal growth factor (EGF) nanoparticles named as (SCPEHG) chitosan nanoparticle EGF hydrogel that may provide both antibacterial and would healing-enhanced functionalities. The EGF encapsulated chitosan nanoparticles were fabricated by using polyanionic as the coacervation crosslink agent. Through the DLS analysis, the size and surface charge of the EGF-loaded nanoparticles are 352 ± 16 nm and 20.5 ± 2.3 mV, respectively. The encapsulation efficiency and the loading rate of the EGF in the nanoparticles were 93.13% and 0.044%. The optimal effective dosages of 24 mM Ag+ and 60 ug/mL EGF for the SCPEHG manufacture were first determined based on the results of antibacterial, cytotoxicity, and cell growth examinations. When the silver ions released in 48 hours, the SCPEHG release were 6.70 mg/L and we can find over 96% cells survival in vitro experiments. The EGF hydrogel release were 1.3 ng/mL in PBS in 48 hours, we also can notice that the cell grow up more fast than in water. We characterized the optimized SCPEHG and found that the composite hydrogel was able to provide sustained release of Ag+ and EGF, and exhibited a significantly higher hydration capacities, including the swelling degree and equilibrium water content, in PBS than those in deionized water, showing that the developed SCPEHG is highly applicable in the ion-rich environment such as burns wound site. In summary, we develop the hydrogel is highly advantageous for curing burns wound and enhancing cell growth.
顯示於類別:	[生物醫學工程研究所 ] 博碩士論文

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