利用原位創新法及機械力化學法合成酵素金屬有機骨架複合材料及其活性比較

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/85783

Title:	利用原位創新法及機械力化學法合成酵素金屬有機骨架複合材料及其活性比較
Authors:	沈玉倢;Shen, Yu-Jie
Contributors:	化學學系
Keywords:	金屬有機骨架材料;酵素固定化;Metal-organic Framworks;Enzyme Immobilization
Date:	2021-08-25
Issue Date:	2021-12-07 11:24:30 (UTC+8)
Publisher:	國立中央大學
Abstract:	金屬有機骨架材料 (Metal-organic Frameworks, MOFs) 近年來成為最熱門的孔洞材料之一，也因具孔洞多樣性、高比表面積以及熱穩定性等多種優勢，使其應用性非常多元化，其中一項應用即為酵素固定化之載體。本實驗室於2015年領導全球，開創出原位創新合成法 (de novo approach)，成功將酵素包覆於MOFs之中，酵素依然保有生物活性，且藉由材料提供的孔洞篩選性得到良好保護，緊接著於2019年再次領先世界，利用機械力化學法 (Mechanochemical method) 將酵素包覆於MOFs之中。上述主要包覆的酵素為過氧化氫?，其具有受質小且不需要輔?的特性，然而在鹼性環境下較易失去活性。為了要讓上述系統能夠應用更多的酵素，因此本研究也包覆了??，其具有較慢的反應速率，在實驗進行時可以更容易觀察催化過程，將??包覆在材料當中，將可成為一個觀察酵素催化的全新平台。本研究成功以機械力化學法合成Enzyme@ZIF-90，並透過各種儀器鑑定材料之特性。雖然對於測量??活性並未得到良好的結果，但同時也發現利用機械力化學法合成的CAT@ZIF-90不但與原位水相合成一樣，酵素保留良好的生物活性，但此法具有更高的產率，使其應用在工業上具有極大的潛力。;Metal-organic frameworks (MOFs) have become one of the most popular porous crystalline materials in recent years. Because of their porous diversity, large specific surface area and thermal stability, they are promising for a wide range of applications, for example enzyme immobilization is one of the attractive areas for our interests. In 2015, our group encapsulated catalase (CAT) enzyme into Zeolitic imidazole framework-90, CAT@ZIF-90, via mild water-based de novo approach, and afterward encapsulated enzymes into MOFs, Enzyme@MOFs, via a mechanochemical method in 2019. One of the mainly above-mentioned enzymes is catalase, which has the characteristics of a small substrate and no need for the coenzyme, but it is easier to lose activity in an alkaline environment. In order to expand the applications of de novo approach, we also encapsulated urease, which has a relatively lower reaction rate that provides a new platform for observing enzyme catalysis. In summary, we successfully synthesized Enzyme@ZIF-90 via a mechanochemical method and identified the characteristics through various instruments. Although urease did not show the expected activity, this method still retains catalase activity and improves the yield. Therefore, it still has a great potential for the further industrial applications.
Appears in Collections:	[Graduate Institute of Chemistry] Electronic Thesis & Dissertation

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