白光干涉術之相位型生物感測器;Phase-Type Biosensor with White Light Interferometry

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题名:	白光干涉術之相位型生物感測器;Phase-Type Biosensor with White Light Interferometry
作者:	李宥霆;Lee, Yu-Ting
贡献者:	光電科學與工程學系
关键词:	白光干涉儀;生物感測器;免疫球蛋白G
日期:	2021-07-21
上传时间:	2021-12-07 11:43:20 (UTC+8)
出版者:	國立中央大學
摘要:	本論文使用掃描式白光干涉儀作為光學轉換器並應用於生物感測器，利用白光干涉技術搭配快速傅立葉轉換法將時域的干涉光強訊號轉換為頻域的傅立葉相位頻譜。利用反射相位作為分析的依據，將生物辨識元件與分析物視為薄膜層，當不同濃度的分析物進行反應時，等效折射率會隨反應濃度的分析物數量而改變，根據相位與折射率的關係，探討分析物在不同濃度時的實驗結果。針對在測量時可能產生的誤差進行調整，其中包含傾斜角度的影響及對反應前後相位差的基準點不同進行改善。在測量結果中，本實驗將最大波數代入擬合的線性方程式中，計算出反應前後相位差的變化量，將此變化量稱為截距差，並以此作為等效折射率變化的標準。採用生醫檢測上的非線性回歸模型，對8個不同濃度的實驗結果進行擬合，驗證實驗結果的合理性，其R平方值為0.9993，利用此模型計算本系統的檢測極限(Limit of Detection)可達到0.019 ng?mL^(-1)。 ;This thesis aims to detect biosensor by using White Light Scanning Interferometer (WLSI) as the optical transducer. The technology which combines White Light Interferometry and Fast Fourier Transform changes the signal of interference light in time domain to the spectrum of Fourier phase in frequency domain. To discuss the result when analyte responses at different concentration, the analysis of experiment is based on the reflective phase and viewing biorecognition element and analyte as two thin films. When analyte is responding with biorecognition element, the effective refractive index of two thin films will change by the amount of analyte at different concentration, then getting result by the relationship between refractive index and phase. Adjusting two possible errors when measuring in experiment, including the effect of tilt and the base point of phase difference before and after reaction are different. In the experimental result, substituting maximum wave number into equation of linear regression to calculate the variable quantities of phase difference before and after reaction. Viewing the variable quantity as the standard of effective refractive index changes, and called the variable quantity as the Intercept Difference. To verify the reasonableness of experimental result uses the nonlinear regression model with bioanalysis to fit the result at eight different concentrations. The R squared is 0.9993, and the limit of detection (LOD) is 0.019 ng?mL^(-1) by calculating from this model.
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