本論文提出以全像光學元件取代傳統數位全像術中的分光鏡以及物鏡的功能,目的要讓無透鏡傅氏轉換數位顯微鏡(Lensless Fourier Transform Digital Holographic Microscope)為基礎架構中的訊號影像可以更靠近電子感光元件,接收更多物體高頻資訊,提升系統之解析度(Resolution)與視野(Field of View,簡稱FOV),並且可以減少擷取影像架構之體積。而我們重建影像之數位對焦原理更是用角譜傳遞法(Angular Spectrum Propagation Method)取代單次傅氏轉換(Fourier Transform),以及解決費耐爾繞射公式(Fresnel Diffraction)與夫朗和斐繞射公式(Fraunhofer Diffraction)有傳遞距離限制的不足,來還原訊號影像。最後以實驗與模擬驗證將架構微型化的可能性,希望在未來可以結合手機與平板等手持裝置,取代物鏡實現縮小化以及商業化的目的。;In this thesis, we proposed to replace beam splitter and objective microscope in the traditional digital holographic configuration with holographic optical element. The purpose is to make the signal image in the configuration based on lensless Fourier Transform Digital Holographic Microscope closes to electronic photosensitive element. It can collect higher frequency signal light and makes image device more compact to improve the resolution or field of view of the system. The digital focusing principle that could reconstructed image is Angular Spectrum Propagation Method instead of Fourier Transform to solve the limitation of propagation distance on Fresnel Diffraction or Fraunhofer Diffraction. Finally, experiments and simulations demonstrates the possibility of the miniaturized structure. We hoped that portable devices such as mobile phones and tablets can be combined to replace the objective microscope to achieve the purpose of miniaturization and commercialization in the future.