近年來光學檢測儀器往不同的方向發展，尤其在生物、醫學方面受到許多的運用，能夠深入了解分子或物質之間的交互作用並進行分析。表面電漿共振對於環境折射率的變化相當靈敏，且具有免標記的特性，應用在生物檢測器上相當具有優勢。 本研究以相位為量測依據，使用共光程的方式，可減少因環境的擾動造成分析相位時的誤差；取出相位的方式是利用項移式干涉儀概念，利用四步相移演算法計算出相位，並搭配上偏振像素攝影機，可以即時獲得空間中的相位分布，利用影像即可辨別出相位的變化程度。實驗將利用甘油水溶液來驗證此系統下，光強式量測與相位式量測的靈敏度與檢測極限，證明了相位式量測比光強式優異了一個數量級，相位式量測的靈敏度為790.63 degree/RIU，檢測極限達到1.4??10?^(-6) RIU；接著改變不同入射角，分析不同入射角下相位式量測的變化，發現當入射角越接近SPR角時，靈敏度變得更高，且檢測極限提升至1.6??10?^(-7) RIU。 ;In recent years, optical detection has developed in different directions, especially in biology and medicine. Surface plasmon resonance (SPR) is an effective method to analyze the interaction between molecules or matter. The SPR technique is very sensitive when the refractive index of the environment is change, and offers label-free detection, which is advantageous when applied to biological detectors, which is advantageous when applied to biological detectors. This study uses phase as the measurement basis, and belongs the common path method to reduce the phase error caused by environmental disturbance. The way to extract the phase is to use the term shift interferometer concept with a polarized pixel camera. The spatial phase-shifting algorithms can be obtained in real time, and the phase images change can be distinguished. The experiment will use glycerin solution to verify the sensitivity and limit of detection (LOD), and proves that the phase measurement is better than the intensity measurement, the phase measurement sensitivity is 790.63 degree/RIU and the is LOD 1.4??10?^(-6) RIU. The incident angles change, caused the phase change, and find that the sensitivity becomes higher when the incident angle is closer to the SPR angle and the LOD increases to 1.6??10?^(-7) RIU.
