組織工程應用之三維曲線列印─ 以皮膚傷口外形為例;3D Curve Printing for Tissue Engineering Applications ── A Case Study of Wound Shapes in Skin

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题名:	組織工程應用之三維曲線列印─ 以皮膚傷口外形為例;3D Curve Printing for Tissue Engineering Applications ── A Case Study of Wound Shapes in Skin
作者:	何佳宜;He, Jia-Yi
贡献者:	機械工程學系
关键词:	組織工程;積層製造;曲線列印;3D生物列印;人工皮膚;Tissue engineering;Additive manufacturing;Curve printing;3D bioprinting;Artificial skin
日期:	2021-06-28
上传时间:	2021-12-07 13:14:44 (UTC+8)
出版者:	國立中央大學
摘要:	常見人工皮膚為大量生成製造而成，於移植過程中需修剪外形以符合病患傷口，如此勢必有殘留部分材料，本論文著重於針對皮膚傷口外形製作出符合該外形之皮膚物件，並以還原皮膚結構加強人工皮膚結構穩固為目標，客製化人工皮膚以減少被修飾的材料損耗。本論文將運用3D生物列印之技術客製化人工皮膚之製作，傳統以水平層積方式印製人工皮膚，但皮膚為曲面構造，印製而成的人工皮膚結構強度會因為其層積方式而下降，為此本論文將使用順著傷口外形之曲線層積路徑列印方式印製人工皮膚。該方法使用固狀底座以符合該傷口底部特徵，將欲印製人工皮膚置於底座之上列印，硬質底座可配合水凝膠的質地如果凍易塌陷特性，保有傷口之外觀特性。藉由X方向切層與Y方向切層所獲得之路徑生成，綜合兩者路徑以組合出網狀結構。並可由使用者對該路徑生成內部數據做細微調整以達使用者所需。生成之路徑可為模擬皮膚結構分別生成真皮層路徑與表皮層路徑，使用水凝膠與兩種細胞混合印製，接續後續的生物實驗。本實驗列印成果可分為兩個，其一以水平面層積方式列印網格狀的鼻子，其二使用曲線層積方式列印人工皮膚，證明該曲線層積列印路徑於不平整表面且能完整將該物件列印成功。發展出四種定位方式，以利傷口支撐底座於列印座標中定位，確認該傷口實際列印位置正確且不發生碰撞。然該成功案例僅限於實心之物件，對於中空物件恐有錯誤產生。在於表皮層之列印成果產生凹洞之非預期狀況，此路徑生成方式仍待改善。 ;Common artificial skin is produced in large quantities, and the shape of the artificial skin needs to be trimmed to fit the patient’s wound during the transplantation process, so there is bound to be some residual material.This thesis focuses on making skin objects conforming to the shape of the skin wound, and aims to restore the skin structure to strengthen the stability of the artificial skin structure.Customized production of artificial skin to reduce the loss of modified materials. This paper will use the technology of 3D bioprinting to make customized artificial skin. However, the skin has a curved structure, and the strength of the printed artificial skin structure will decrease due to its layering method. For this reason, this paper will print artificial skin using the printing method of curve layered path that follows the shape of the wound. This method uses a solid base to match the characteristics of the bottom of the wound, and places the artificial skin to be printed on the base. The hard base can be matched with the texture of the hydrogel, which is easy to collapse like a jelly, and retains the appearance of the wound. Generate the path obtained by cutting in the X direction and the Y direction, combining the two paths to form a network structure. And the user can make fine adjustments to the internal data generated by the path, which has reached the user′s needs. The generated path can be to simulate the skin structure to generate the dermis path and the epidermis path respectively, using a mixture of hydrogel and two kinds of cells to print, and continue the subsequent biological experiments. The printing results of this experiment can be divided into two, the first is to print the grid-like nose in the horizontal layering method, and the second is to use the curve layering method to print the artificial skin, which proves the printing path of the curved layer base which can be printed successfully on uneven surfaces. Four positioning methods have been developed to facilitate the positioning of the wound support base in the printing coordinates to confirm that the actual printing position of the wound is correct and does not collide. However, the successful case is limited to solid objects, and there may be errors in hollow objects. Because of the unexpected situation that the printing result of the skin layer produces cavities, the path generation method still needs to be improved.
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