博碩士論文 983204020 詳細資訊



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姓名 林建翰(Chien-Han Lin)  查詢紙本館藏   畢業系所 化學工程與材料工程學系
論文名稱 利用表面化學改質之奈米層狀結構矽基材晶片與四氧化三鐵磁性奈米粒子以提升磷酸化胜肽於質譜儀檢測之靈敏度之研究
(Enhancement of Mass Spectrometry Sensitivity on Phosphopeptide Using Chemical Modified Nanofilament Silicon (nSi) Chip and Iron(II, III) Oxide Magnetic Nanoparticles)
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摘要(中) 近年來，透過組織工程，許多研究團隊於再生醫學新技術有了重大突破，以幹細胞修復受損組織成功的案例也逐年增加。然而幹細胞是否能應用於臨床治療，取決於適當的細胞調控，其和信號傳遞過程中伴隨的蛋白質磷酸化有著密不可分的關係。不幸的是，磷酸化蛋白質在細胞樣本內含量極低，分析前複雜的純化程序往往也在所難免。
質譜儀可對未知生物分子提供珍貴的質荷比之定性與定量資訊，向來是蛋白質體學實驗及生物分析領域上一大利器。近年研究發現奈米結構多孔矽具有吸收紫外光雷射能力，作為輔助脫附游離的介質具有高靈敏度的表現，且在分析小分子樣本時不受基質所干擾。本研究成功研發出以多孔矽改良之奈米層狀結構矽（nanofilament silicon, nSi）質譜分析晶片作為基材，並以特定官能基如羧基、胺基、苯環或金屬親合等接枝於表面，直接分離樣本胜肽。再結合Fe3O4/TiO2 與Fe3O4/ZnO 核/殼結構奈米粒子，藉其表面與磷酸根之專一性吸附更可辨識出β-casein digest中的磷酸化胜肽，且偵測極限可達10-9 M以下。此法可簡化複雜的磷酸化胜肽純化程序並增加質譜分析的效能與選擇性，將可使幹細胞與再生醫學研究效率大幅提升。
摘要(英) In recent year, many research groups have made breakthrough in regenerative medicine through tissue engineering. The number of successful cases using stem cell to repair the damage organ also increases year by year. A appropriate cell regulations facilitate stem cell for possible application to clinical treatment, and the regulation deeply related to protein phosphorylation in cell transduction pathway. Unfortunately, low abundance of phosphorylated peptides and proteins in cell makes identification of phosphorylation sites difficult; hence a complicated purification process prior to analysis is usually unavoidable.
Mass spectrometry (MS) is a useful tool to identify unknown biomolecules qualitatively and quantitatively in proteomics and other related bioanalysis field because it provides valuable mass to charge (m/z) information. In recent researches, silicon-based nanostructure are used as UV absorbing media to ionize biomolecules and show high sensitivity on low molecular weight biological samples without matrix background. In this study, we used nanofilament silicon (nSi) chips as a base which graft the different functional groups onto the chip surface such as carboxyl, amino, phenyl or immobilized metal affinity. We found those modification could separate sample peptides specificly. Phosphopeptide derived from β-casein digest has also been detected by nSi combining with Fe3O4/TiO2 and Fe3O4/ZnO core/shell nanoparticles, and the detection limit could reach 10-9 M. These approaches enable us to simplify the purification process of phosphorylated peptides and proteins, increase the selectivity and sensitivity of MS analysis, and make stem cell and regenerative medicine research more efficient.
關鍵字(中) ★ 表面化學改質
★ 奈米層狀結構矽基材晶片
★ 表面輔助雷射脫附質譜儀
★ 磁性奈米粒子
★ 磷酸化胜肽		 關鍵字(英) ★ nanofilament silicon (nSi) chip
★ SALDI-MS
★ magnetic nanoparticles
★ phosphopeptide
★ surface modification
論文目次 摘要 I
Abstract II
目錄 IV
圖目錄 IX
表目錄 XVI
第一章　緒論 1
1.1　研究背景 1
1.2　研究目的 2
1.3　論文架構 5
第二章　文獻回顧 6
2.1　軟式游離法之發展 6
2.1.1　電噴灑游離法－ESI 6
2.1.2　基質輔助雷射脫附游離法－MALDI 7
2.1.3　表面增強雷射脫附游離法－SELDI 9
2.1.4　表面輔助雷射脫附游離法－SALDI 12
2.2　矽基材雷射脫附游離法－DIOS 15
2.2.1　DIOS之離子化機制 15
2.2.2　DIOS之表面化學改質 18
2.2.2.1　矽基材表面改質法 18
2.2.2.2　表面改質對DIOS-MS之影響 24
2.2.3　DIOS之結構改良與最佳化 29
2.3　磷酸化蛋白質之分析 33
2.3.1　磷酸化蛋白質簡介 33
2.3.2　磷酸化蛋白質樣本分析前處理 35
2.3.3　磷酸化蛋白質樣本純化 37
2.3.3.1　免疫沉降法 38
2.3.3.2　化學衍生法 38
2.3.3.3　金屬親合法 39
2.3.4　磷酸化蛋白質之質譜儀檢測應用 41
2.3.4.1　與質譜儀結合之純化法 41
2.3.4.2　蛋白質定序與磷酸化位置辨識 44
第三章　材料與方法 47
3.1　實驗設備 47
3.2　實驗藥品 48
3.3　實驗方法 51
3.3.1　矽晶片表面蝕刻 51
3.3.2　nSi Chip表面化學改質 53
3.3.2.1　NH2正電性官能基表面改質法 54
3.3.2.2　COOH負電性官能基表面改質法 55
3.3.2.3　Phenyl疏水性官能基表面改質法 56
3.3.2.4　Phenyl-NH2疏水性正電官能基表面改質法 57
3.3.2.5　Phenyl-COOH疏水性負電官能基表面改質法 58
3.3.2.6 帶有Ni離子的IMA-Ni2+表面改質法[98-100] 59
3.3.2.7　測試nSi Chip改質成果與對混合胜肽分離之效果 60
3.3.3　Fe3O4/TiO2 核/殼結構磁性奈米粒子合成[14] 61
3.3.4　Fe3O4/ZnO 核/殼結構磁性奈米粒子合成[15] 64
3.3.5　高靈敏度nSi Chip無基質質譜分析法 66
3.3.6　nSi Chip結合奈米粒子之質譜分析法 67
3.3.7　甲醇對nSi Chip離子化能力影響 69
3.3.8　Citric Buffer對nSi Chip質譜效能影響 70
第四章　結果與討論 72
4.1　nSi Chip表面化學改質 72
4.1.1　AFM驗證 72
4.1.2　ESCA驗證 75
4.1.2.1　鍍金 75
4.1.2.2　NH2表面改質 76
4.1.2.3　COOH表面改質 77
4.1.2.4　Phenyl表面改質 78
4.1.2.5　Phenyl-NH2 表面改質 79
4.1.2.6　Phenyl-COOH表面改質 80
4.1.2.7　IMA-Ni2+表面改質 81
4.1.3　SELDI-MS驗證 82
4.1.3.1　鍍金與浸泡甲醇影響 84
4.1.3.2　NH2表面改質 88
4.1.3.3　COOH表面改質 91
4.1.3.4　Phenyl表面改質 94
4.1.3.5　Phenyl-NH2表面改質 97
4.1.3.6　Phenyl-COOH表面改質 100
4.1.3.7　IMA-Ni2+ 表面改質 103
4.2　Fe3O4@TiO2與Fe3O4@ZnO奈米粒子應用 105
4.2.1　SEM觀測 105
4.2.2　Citric Buffer對nSi效能之影響 108
4.2.3.1　以Peptide Mixture測試純化與質譜能力 110
4.2.3.2　以β-casein測試純化與質譜能力 114
4.2.4　Fe3O4@ZnO 奈米粒子純化效果試驗 117
4.2.4.1　以Peptide Mixture測試純化與質譜能力 117
4.2.4.2　以β-casein測試純化與質譜能力 121
第五章　結論與未來展望 124
參考文獻 126
附錄 140
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指導教授 陳文逸(Wen-Yih Chen) 審核日期 2011-6-22
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