探討光品質對於Aspergillus terreus生產衣康酸之影響

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：26

、訪客IP：18.118.205.146

姓名

廖偉修(Wei-hsiu Liao) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

探討光品質對於Aspergillus terreus生產衣康酸之影響
(Effect of light quality on the production of itaconic acid by Aspergillus terreus in batch culture)

相關論文

★ 探討菌體形態對於裂褶菌多醣體之影響	★ 探討不同培養方式對猴頭菇抗氧化與抗腫瘤性質的影響
★ 探討不同培養溫度Aspergillus niger 對丹參之機能性影響	★ 光合菌在光生物反應器產氫之研究
★ 探討培養溫度對巴西蘑菇液態醱酵之影響	★ 利用批式液態培養來探討檸檬酸對裂褶菌生長及其多醣體生成影響之研究
★ 探討不同培養基組成對光合菌Rhodobacter sphaeroides生產Coenzyme Q10之研究	★ 利用混合特定菌種生產氫氣之研究
★ 探討氧化還原電位作為Clostridium butyricum連續產氫之研究	★ 探討培養基之pH值與Xanthan gum的添加對巴西蘑菇多醣體生產之影響
★ 探討麩胺酸的添加和供氧量對液態發酵生產裂褶菌多醣體之研究	★ 探討以兩水相系統提昇Clostridium butyricum產氫之研究
★ 探討通氣量對於樟芝醱酵生產生物鹼之影響	★ 探討深層發酵中環境因子對巴西洋菇生產多醣之影響
★ 探討通氣量對於樟芝發酵生產與純化脂解酵素之研究	★ 探討以活性碳吸附酸來提昇Clostridium butyricum產氫之研究

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

[檢視]

[下載]

本電子論文使用權限為同意立即開放。
已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。
請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。

摘要(中)

衣康酸由於其獨特的結構特性，因此廣泛地被利用在人造纖維、樹脂與其他合成材料上。而土麴黴 Aspergillus terreus 由於具有較高的衣康酸產率，因此成為許多研究機構討論的重點之一。由於過去文獻對於Aspergillusterreus 之研究主要是針對溫度、pH 值與通氣量等進行探討，對於光照的影響著墨不多，因此本研究遂以光照條件做為實驗之主軸。
本研究首先找出實驗所用菌株Aspergillus terreus BCRC 31128 之最適發酵溫度40℃，再設計以避光的0 Lux 為對照組，並探討在白光強度300、500、800 及1000 Lux，以及紅光與藍光150、300 及500 Lux 的條件下，對於衣康酸發酵的影響。
由實驗結果顯示，在白光強度500 Lux 以下的條件，對於衣康酸產量有正面幫助，但當白光強度高於800 Lux 時，則會對菌體的生長有抑制性。而低強度的紅光與藍光，雖然對菌重的生成沒有顯著影響，但皆比白光有較佳的刺激衣康酸生產效果；最佳之光照條件為強度300 Lux 的紅光，於此條件下，衣康酸產量比起避光條件要提升108 ％，同時產物對基質的轉化率提升97.5 ％。
本實驗發現單一波長之光源對於刺激Aspergillus terreus 生產衣康酸有較佳的效果，而紅光條件又優於藍光條件。

摘要(英)

Itaconic acid, also called methylene succinic acid, is an important monomer for the manufacturing of resins, fibers, plastic, or other polymeric materials and has been widely utilized in chemical industries.
Aspergillus terreus is one of the best itaconic acid producer because of its high yield. In past scientific literature, some experiment conditions, for instance,
pH and temperature, are investigated. However, the investigation of light conditions for itaconic acid fermentation is deficient. For this reason, the study
of mine discussed the effect on light quality for itaconic acid fermentation.
In the study, the first step I found the best fermentation temperature is 40℃ by Aspergillus terreus BCRC 31128. Second, I devised the conditions, as the lack of light (0 Lux), and the conditions of 300, 500, 800, and 1000 Lux of white light. After all of the experiment of white light, I used red light and blue light, the conditions as 150, 300, and 500 Lux, and then discussed the results of all experiment.
From the results, the yield of itaconic acid increased with the white light intensity below 500 Lux, and decreased with the white light intensity upon 800 Lux. The best light condition of itaconic acid fermentation is red light with intensity as 300 Lux, the amount of itaconic acid is 108％ more than the condition of 0 Lux.

關鍵字(中)

★ 光接受器
★ 衣康酸
★ 光波長
★ 光強度
★ 土麴黴

關鍵字(英)

★ Light wavelength
★ Light intensity
★ Light acceptor
★ Itaconic acid
★ Aspergillus terreus

論文目次

摘要 ...................................................I
Abstract ...............................................II
誌謝 ...................................................III
目錄 ...................................................IV
圖目錄 .................................................VII
表目錄 .................................................IX
第一章、緒論 ............................................1
1-1 研究動機 ........................................... 1
1-2 研究目的 ........................................... 2
1-3 研究架構 ........................................... 3
第二章、文獻回顧 ....................................... 4
2-1 衣康酸 ............................................. 4
2-1.1 衣康酸簡介 ....................................... 4
2-1.2 衣康酸來源 ....................................... 5
2-1.3 衣康酸之應用與市場 ............................... 7
2-2 真菌 ............................................... 9
2-2.1 真菌簡介 ......................................... 9
2-2.2 真菌的分類 ....................................... 10
2-2.3 土麴黴 (Aspergillus terreus) 之簡介 .............. 11
2-3 土麴黴發酵生產衣康酸之生物合成途徑 ................. 13
2-4 影響衣康酸產率之因素 ............................... 16
2-4.1 培養基組成 ....................................... 16
2-4.2 pH 值 ............................................ 18
2-4.3 通氣量與攪拌速率 ................................. 19
2-4.4 溫度 ............................................. 20
2-5 光對生物發酵之影響 ................................. 21
2-6 光接受器 ........................................... 24
2-6.1 光敏素 (Phytochrome) ............................. 24
2-6.2 隱花色素 (Cryptochrome) .......................... 26
第三章、實驗材料與方法 ................................. 27
3-1 實驗規劃 ........................................... 27
3-2 實驗材料 ........................................... 28
3-2.1 實驗菌株 ......................................... 28
3-2.2 實驗藥品 ......................................... 29
3-2.3 實驗儀器與設備 ................................... 30
3-3 實驗方法 ........................................... 32
3-3.1 菌種保存 ......................................... 32
3-3.2 培養基組成 ....................................... 32
3-3.3 實驗操作方法與條件 ............................... 35
3-4 分析方法 ........................................... 40
3-4.1 發酵液處理流程圖 ................................. 40
3-4.2 菌重分析 ......................................... 40
3-4.3 葡萄糖殘量分析 ................................... 41
3-4.4 衣康酸含量分析 ................................... 43
第四章、結果與討論 ..................................... 45
4-1 最適發酵溫度實驗 ................................... 45
4-2 白光強度對Aspergillus terreus 發酵生產衣康酸之影響 . 51
4-2.1 搖瓶培養實驗 ..................................... 51
4-2.2 發酵槽實驗 ....................................... 54
4-3 不同波長光源對Aspergillus terreus 發酵生產衣康酸之影響 ..................................................... 58
第五章、結論與建議 ..................................... 66
5-1 結論 ............................................... 66
5-2 建議 ............................................... 67
第六章、參考文獻 ....................................... 68

參考文獻

林玉蕙，「衣康酸生合成相關之研究」，國立陽明大學生物化學研究所，博士論文，2004。
孫楷惠，「隱球菌藍光訊息傳導分子機制之探討」，國立臺灣大學植物病理與微生物學研究所，碩士論文，2004。
彭鉦欽，「探討不同光強度對Penicillium brevicompactum 在液態發酵中生產Mycophenolic acid 之影響」，國立中央大學化學工程與材料工程研究所，碩士論文，2006。
黃俊凱，「探討光照對Saccharomyces cerevisiae 生產乙醇之影響」，國立中央大學化學工程與材料工程研究所，碩士論文，2008。
張彥華，「探討光照對Aureobasidium pullulans生產胞外多醣之影響」，國立中央大學化學工程與材料工程研究所，碩士論文，2009。
劉圈烯、何雲、齊勝利、王成章、陳繼紅、潘俊良、王彥準，「光敏色素研究進展」，中國農學通報，第21 卷第5 期，2005。
吳國芳、馮志堅，「植物學」，高等教育出版社，2000。
景明化工物質安全資料表，景明化工股份有限公司，2011。
生物資源線上目錄，食品所生物資源保存及研究中心，2011。
Ahmad, M. and Cashmore, A. R., “HY4 gene of A. thaliana encodes a protein characteristics of a blue light photoreceptor”, Nature. 366, pp. 162-166, 1993.
Alfredo, H. E. and Benjamin, A. H., “Looking through the eyes of fungi：molecular genetics of photoreception”, Molecular Microbiology. 64, pp. 5-15, 2007.
Anne, B., Kay, V., Ronja, T., Janina, P., Daniel, V., Nicole, F. D., and Reinhard, F., “The Aspergillus nidulans phytochrome fpha represses sexual development in the red light”, Current Biology. 15, pp. 1833-1838, 2005.
Baup, S., “Ueber eine neue Pyrogen-Citronensaure”, U. S. W. Annalen 19, pp. 29-38, 1836.
Bennett, J. W. and Klich, M. A., “Aspergillus : Biology and industrial applications”, Butterworth-Heinemann Press, pp. 91-124, 1992.
Bentley, R. and Thiessen, C. P., “Biosynthesis of itaconic acid in Aspergillus terreus. I. Tracer studies with 14C-labelled substrates”, J. Biol. Chem. 226, pp. 673-687, 1957.
Bentley, R. and Thiessen, C. P., “Biosynthesis of itaconic acid in Aspergilleus terreus. II. Early stages in glucose dissimilation and the role of citrate”, J. Biol. Chem. 226, pp. 673-688, 1957.
Bentley, R. and Thiessen, C. P., “Biosynthesis of itaconic acid in Aspergilleus terreus. III. The properties and reaction mechanism of cis-aconitic decarboxylase”, J. Biol. Chem. 226, pp. 689-701, 1957.
Bonnarme, P., Gillet, B., Sepulchre, A. M., Role, C., Beloeil, J. C., and Ducrocq, C., “Itaconate biosynthesis in Aspergilleus terreus“, J. Bacteriology. 177, pp. 3573-3578, 1995.
Briggs, W. R. and Huala, E., “Blue-light photoreceptors in high plants”, Annu. Rev. Cell Dev. Biol. 15, pp. 33-62, 1999.
Calam, C. T., Oxford, A.E., and Raistrick, H., “Studies in the biochemistry of microorganisms. LXIII. Itaconic acid, a metabolic product of a strain of Aspergillus terreus”, Thom. Biochem. J. 33, pp. 1488-1495, 1939.
Cros, P. and Schneider, D., French patent 8805487, 1988.
Eimhjellen, K. E. and Larsen, H., “The mechanism of itaconic acid formation by Aspergillus terreus. II. The effect of substrates and inhibitors”, Biochem. J. 60, pp. 139-147, 1955.
Elizabeth, E. L., “Effect of light regimens and intensities on morphogenesis of the discomycete Pyronema domesticum”, Mycologia. 71, pp, 699-712, 1979.
Griffith, G. W., Jenkins, G. I., Milner-White, E. J., and Clutterbuck, A. J.,“Homology at the amino acid level between plant phytochromes and a regulator of asexual sporulation in Asoergillus nidulans”, Photochem. Photobiol. 59, pp. 252-256, 1994.
Gyamerah, M. H., “Factors affecting the growth from of Aspergillus terreus NRRL 1960 in relation to itaconic acid fermentation”, Appl. Microbiol. Biotechnol. 44, pp. 356-361, 1995 I.
Gyamerah, M. H., “Oxygen requirement and energy relations of itaconic acid fermentation by Aspergillus terreus NRRL 1960”, Appl. Microbiol. Biotechnol. 44, pp.20-26, 1995 II.
Harding, R. W. and Melles, S., “Genetic analysis of phototropism of Neurospora crassa perithrcial beaks using white collar and albino mutants”, Plant Physiol. 72, pp. 996-1000, 1983.
Karu, T. I., Kutomkina, E. V., Lyapunova, T. S., and Pomoshnikova, V.A., “The activation of yeast metabolism with He-Ne laser radiation. III. Protein synthesis in Saccharomycodes ludwigii grown in aerobic and an aerobic condition”, Laser life sci. 5, pp. 259-266, 1993.
Kautola, H., Vahvaseleka, M., Linko, Y.Y., and Linko, P., ”Itaconic acid production by immobilized Aspergillus terreus from xylose and glucose”, Biotechnol. Lett. 7, pp. 167-172, 1985.
Kendrick, R. E. and Kronenberg, G. H. M,“Photomorphogenesis in Plants”, 1986.
Kobayashi, T., Ven. Dedem, G., and Moo-Young, Y., “Oxygen transfer into mycelial pellets”, Biotechnol. Bioeng. 15, pp. 27-45, 1973.
Kinoshita, K., “Ueber eine neue Aspergillus Art, Aspergillus itaconicus”, Bot. Mag. 45, pp. 45-61, 1931.
Lai, L. S., Hung, C. S., and Lo, C. C., “Effects of lactose and glucose on production of itaconic acid and lovastatin by Aspergillus terreus ATCC 20542”, J. Bioscience and Bioengineering. 104, pp. 9-13, 2007.
Linden, H. and Macino, G., “White collar 2, a partner in blue-light singal transduction,controlling expression of light-regulated genes in Neurospora crassa”, EMBO J. 16, pp. 98-109, 1997
Lockwood, L. B. and Nelson, G. E. N., “Some factors affecting the production of itaconic acid by Aspergillus terreus in agitated cultures”, Arch. Biochem. 10, pp. 365-374, 1946.
Lockwood, L. B. and Schweiger, L. B., “Microbiol Technology. VIII. Citric acid and itaconic acid fermentation”, Academic Press, pp. 183-199, 1971.
Miller, G. L., “Use of dinitrosalycilic acid reagent for determination of reducing sugars”, Anal. Chem. 31, pp. 426-430, 1959.
Nelson, G. E. N., Traufler, D. H., Kelley, S. E., and Lockwood, L. B., “Production of itaconic acid by Aspergillus terreus in 20-liter fermenrators”, Ind. Eng. Chem. 44, pp. 1166-1168, 1952.
Pfeifer, V. F., Vojnovich, C., and Heger, E. N., “Itaconic acid by fermentation with Aspergillus terreus”, Ind. Eng. Chem. 44, pp. 2975-2980, 1952.
Quail, P. H., Boylan, M. T., Parks, B. M., Short, T. W., Xu, Y., and Wagner, D., “Phytochromes: photosensory perception and signal transduction”, Science. 268, pp. 675-680, 1995.
Roehr, M. and Kubicek, C.P., “Further organic acids. In : Rehm, H.J., Reed, G. (Eds.), Biotechnology”, Products of Primary Metabolism. 6, second ed. VCH Weinheim, pp. 364-379, 1996.
Roukas, T., “Influence of impeller speed on citric acid production and selected enzyme activities of TCA cycle”, Soc. Ind. Microbiol. 7, pp. 221-226, 1991.
Rychestra, M. and John, W. D. A., “The growth of Aspergillus terreus and the production of itaconic acid in bath and continuous culture. The influences of pH”, J. Chem. Biotechnol. 31, pp. 509-512, 1981.
Sargent, M. L. and Briggs, W. R., “The effect of light on a circadian rhythm of condiation in Neurospora”, Plant Physiol. 42, pp. 1504-1510, 1993.
Shimi, I. R. and Nour El Dein, M. S., “Biosynthesis of itaconic acid by Aspergillus terreus”, Arch. Mikrobiol. 44, pp. 181-188, 1962.
Starostzik, C. and Marwan, W., “A photoreceptor with charac-teristics of a phytochrome triggers sporulation in the true slime mould Physarum polycephalum”, FEBS Lett. 370, pp. 146-148, 1995.
Tsuyoshi, M., Akira, M., Toshie, K., Tadashi, O., Yasuaki, U., Fumihiro S., Hiroyuki, S., Akira, W., and Masahiro, K., “Light effect on cell development and secondary metabolism in Monascus”, J. Ind. Microbiol. Biotechnol. 32, pp. 103-108, 2005.
Willke, T. and Vorlop, K. D., “Biotechnological production of itaconic acid”, Appl. Microbiol. Biotechnol. 56, pp. 289-295, 2001.
Winskill, N., “Tricarboxylic acid cycle activity in relation to itaconic acid biosynthesis by Aspergillus terreus”, J. General. Microbiol. 129, pp. 2877-2883, 1983.
Yahiro, K., Shibata, S., Park, Y., and Okabe, M., “Effect itaconic acid production from raw corn starch”, J. Ferment. Bioeng. 84, pp. 375-377, 1997.
Yano, T., Kodama, T., and Yamada, K., “Fundamental studies on the aerobic fermentation. Part VIII. Oxygen transfer within a mold pellet”, Agric. Biol. Chem. 25, pp. 580-584, 1961.

指導教授

徐敬衡(Chin-hang Shu)

審核日期

2011-7-21

推文