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姓名	陳郁融(Yu-jung Chen)  查詢紙本館藏	畢業系所	環境工程研究所
論文名稱	介電質放電對大腸桿菌及枯草芽孢桿菌滅菌效率與機制之初步探討 (Preliminary Investigation on the Sterilization of B. subtilis and E. coli Using DBD Plasma)
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摘要(中)	如何加快細菌死亡或使其快速失去繁殖能力且不對人體及材料造成危害為現今熱烈討論的研究議題之一。電漿應用於滅菌技術發展至今已有一定成效且與傳統滅菌技術相比，其對材料本身結構無影響也無毒害物質殘留，因此本研究針對介電質放電以放電參數及不同氣體電漿對滅菌效率及滅菌機制進行初步探討，以期開發更安全有效的滅菌技術。首先針對不同放電參數進行探討發現氣流流動方向會影響介電質放電滅菌的殺菌效果。而不同種類的氣體電漿，其殺菌效果也各有不同，本研究以氬氣、氮氣、氧氣及1％四氟甲烷與空氣電漿殺菌效果進行探討，結果為添加如氧氣或四氟甲烷等負電性氣體入氣體電漿系統能提升殺菌效果。以光學放射光譜儀分析物種，空氣電漿中O(2D0)及O(5P0)能與臭氧一同氧化細胞壁，而氮氣 − 氧氣電漿生成氧化力強的NOγ能有效提升殺菌效果。掃描式電子顯微鏡及金相顯微鏡得到滅菌前後的細菌形態，隨著滅菌時間增加其細菌數目隨之減少且細胞壁皆呈現破裂情形，從中了解電漿能將表面細菌以慢燃方式移除，強氧化力的活性物種氧化破壞細菌細胞壁，其細胞壁模糊且變形。蛋白質及總糖分析中發現影響蛋白質釋出量最主要為臭氧等活性物種，影響總糖釋出量為電漿生成的電荷。研究結果中顯示電漿滅菌時生成的電荷與活性物種同時存在且與細菌作用，主要以電荷直接轟擊作用促使電漿區內的細胞壁破裂，而活性物種以氧化作用輔助電漿加速破壞細胞壁，促使細菌水解、死亡。
摘要(英)	How to develop a safe and efficient sterilization method has become an emerging topic in the 21th century. Although conventional methods like heating, ethylene oxide (EtO) or ultraviolet (UV) can achieve good germicidal effect, they are time- and energy-consuming. Compared to conventional methods, nonthermal plasma has been widely used for sterilization of microorganisms not only in food processing, but also in health-care industry. There are many advantages of applying nonthermal plasma for sterilization. In this study, we focus on the germicidal effect of plasma sterlization and the mechanism associated of dielectric barrier discharge (DBD) plasma. Among the parameters investigeted, gas flow patterns would affect sterilization efficiency achieved with DBD and different gas compositions result in different germicidal effect. In single gas plasma, the best germicidal effect is achieved with oxygen. However, addition of oxygen into other working gases also enhances the germicidal effect. The best one is achieved with the gas composition of Ar 75%/O2 25% in mixture. From species analysis, NOγ produced by N2-O2 plasma, as well as O(2D0) and O(5P0) produced by air plasma can oxidize the cell wall of bacteria. SEM and optical microscopy images indicate that samples treated by plasma, ozone results in hydrolysis and oxidation, respectively. Protein and total sugar analysis both show that the degrees of damage to the cell wall caused by different treatment methods vary. The mechanism of cell wall damaged by DBD involves with direct charge bombardment, and then ozone assists the charge to oxidize the cell wall, resulting in rupture.
關鍵字(中)	★ 介電質放電 ★ 殺菌效果 ★ 滅菌機制 ★ 臭氧 ★ 總糖及蛋白質釋出量 ★ 細胞壁破壞程度	關鍵字(英)	★ Total sugar and protein releasing amount ★ Ozone ★ Sterilizaiton mechanism ★ Germicidal effect ★ DBD ★ Damage of cell wall
論文目次	摘要 I ABSTRACT II 誌謝 IV 目錄 V 表目錄 IX 圖目錄 X 第一章 前言 1 1-1 研究緣起 1 1-2 研究目的 2 第二章 文獻回顧 3 2-1 病原體對人體的危害 3 2-1-1 細菌 4 2-1-2 革蘭氏陽性菌 5 2-1-3 革蘭氏陰性菌 6 2-2 滅菌方法介紹 6 2-3 電漿基本原理 10 2-4 電漿種類 10 2-5 非熱電漿之種類與特性 12 2-5-1 輝光放電 13 2-5-2 電暈放電 14 2-5-3 介電質放電 15 2-5-4 高週波放電 17 2-5-5 微波電漿 17 2-6 電漿滅菌原理 18 2-6-1 低壓電漿滅菌 19 2-6-2 常壓電漿滅菌 22 2-6-3 細菌於懸浮態或附著態的影響 25 2-6-4 不同電漿源滅菌影響 26 2-6-5 臭氧殺菌 29 2-6-6 水氣對於常壓電漿的影響 30 2-6-7 不同氣體電漿的滅菌比較 30 第三章 材料與方法 33 3-1 實驗儀器與設備 33 3-2 實驗材料 39 3-3 其他相關設備及材料 42 3-4 實驗方法 43 3-4-1 反應器放電基本特性 44 3-4-2 微生物培養與計數 44 3-4-3 介電質放電滅菌測試 46 3-4-4 水氣對於滅菌測試之影響 49 3-4-5 介電質放電生成之臭氧對滅菌之影響 50 3-4-6 不同氣體電漿的滅菌效率 50 3-4-7 紫外光滅菌的影響 51 3-4-8 物種分析 51 3-4-9 細胞形態 52 3-4-10 蛋白質裂解及總糖分析 54 3-5 數據分析 56 3-5-1 臭氧濃度換算 56 3-5-2 波形及功率計算 56 3-5-3 滅菌效率 57 3-5-4 蛋白質及總糖釋出量計算方法 58 3-5-5 各滅菌方法之成本效益計算方法 59 第四章 結果與討論 60 4-1 反應器放電基本特性 60 4-1-1 調控電壓及電流 60 4-1-2 調控頻率 63 4-1-3 調控氣流量 65 4-1-4 調控間距 68 4-1-5 波形及功率 70 4-2 介電質放電滅菌 71 4-2-1 滅菌初始測試 72 4-2-2 調整功率測試 77 4-2-3 調整頻率測試 79 4-2-4 調整氣流流量測試 81 4-2-5 調整時間間隔測試 82 4-3 水氣對於介電質放電滅菌的影響 83 4-4 臭氧對於滅菌的影響 84 4-5 不同氣體種類對介電質放電滅菌的影響 86 4-5-1 常壓氬氣電漿滅菌 87 4-5-2 常壓氮氣電漿滅菌 88 4-5-3 常壓氧氣電漿滅菌 88 4-5-4 常壓氬氣卅氧氣電漿滅菌 89 4-5-5 常壓氮氣卅氧氣電漿滅菌 91 4-5-6 常壓四氟甲烷卅氧氣電漿滅菌 92 4-6 紫外光滅菌 97 4-7 物種分析 98 4-7-1 常壓空氣電漿 98 4-7-2 常壓氧氣電漿 99 4-7-3 常壓氬氣卅氧氣電漿 100 4-7-4 常壓氮氣卅氧氣電漿 101 4-7-5 常壓四氟甲烷卅氧氣電漿 102 4-8 介電質放電滅菌後的細菌形態 111 4-9 蛋白質裂解及總糖分析 116 4-10 介電質放電滅菌機制 119 4-11 各滅菌方法成本效益之比較 122 第五章 結論與建議 124 5-1 結論 124 5-2 建議 125 參考文獻 126
參考文獻	Akitsu, T., Ohkawa, H., Tsuji, M., Kimura, H. and Kogoma, M., “Plasma Sterilization Using Glow Discharge at Atmospheric Pressure”, Surface and Coatings Technology, 193(2005):29-34 Becker, K.H., Kogelschatz, U., Schoenbach, K.H. and Barker, R.J., “Non-Equilibrium Air Plasmas at Atmospheric Pressure”, IOP publishing Ltd., 2005 Bellan, P.M., “Fundamentals of Plasma Physics”, Cambrige University Press, 2006 Boscariol, M.R., Moreira, A.J., Mansano, R.D., Kikuchi, I.S. and Pinto, T.J.A., “Sterilization by Pure Oxygen Plasma and by Oxygen-Hydrogen Peroxide Plasma: An Efficacy Study”, International Journal of Pharmaceutics, 353(2008):170-175 Brelles-Mariño, G., “Biological and Environmental Applications of Gas Discharge Plasmas”, Nova Science Publishers, Inc., 2010 Burlica, R., Grim, R.G., Shin, K.-Y., Balkwill, D. and Locke, B.R., “Bacteria Inactivation Using Low Power Pulsed Gliding Arc Discharge with Water Spray”, Plasma Processes and Polymers, 7(2010):640-649 Capitelli, M. and Bardsley, J.N., “Nonequilibrium Process in Partially Ionized Gases”, Plenum Press, 1990 Chau, T. T., Kao, K.C., Blank, G. and Madrid, F., “Microwave Plasmas for Low Temperature Dry Sterilization”, Biomoteriols, 17(1996):1273-1277 Chen, C.W., Lee, H.M. and Chang, M.B., “Influence of pH on Inactivation of Aquatic Microorganism with a Gas–Liquid Pulsed Electrical Discharge”, Journal of Electrostatics, 67(2009):703-708 Chiang, M.H., Wu, J.Y., Li, Y.H., Wu, J.S., Chen, S.H. and Chang, C.L., “Inactivation of E. coli and B. subtilis by a Parallel-Plate Dielectric Barrier Discharge Jet”, Surface and Coatings Technology, 204(2010):3729-3737 Choi, J.H., Han, I., Baik, H.K., Lee, M.H., Han, D.W., Park, J.C., Lee, I.S., Song, K.M. and Lim, Y.S., “Analysis of Sterilization Effect by Pulsed Dielectric Barrier Discharge”, Journal of Electrostatics, 64 (2006):17-22 Cowan, M.K. and Talaro, K.P., “Microbiology: A System Approach”, McGrawHill Companies, 2009 Deng, S., Cheng, C., Ni, G., Meng, Y. and Chen, H., “Bacillus Subtilis Devitalization Mechanism of Atmohphere Pressure Jet”, Current Applied Physics, 10(2010):1164-1168 Ekem, N., Akan, T., Akgun, Y., Kiremitci, A., Pat, S. and Musa, G., “Sterilization of Staphylococcus Aureus by Atmospheric Pressure Pulsed Plasma”, Surface and Coatings Technology, 201 (2006): 993-997 Eliasson, B. and Kogelschatz, U., “Nonequilibrium Volume Plasma Chemical Processing”, IEEE Transactions on Plasma Science, 19(1991):1063-1077 Eliasson, B., Hirth, M. and Kogelschatz, U., “Ozone Synthesis from Oxygen in Dielectric Barrier Discharges”, Journal of Physics D Applied Physics, 20(1987):1421-1437 Fridman, G., Brooks, A.D., Balasubramanian, M., Fridman, A., Gutsol, A., Vasilets, V.N., Ayan, H. and Friedman, G., “Comparison of Direct and Indirect Effects of Non-Thermal Atmospheric-Pressure Plasma on Bacteria”, Plasma Processes and Polymers, 4(2007):370-375 Gweon, B., Kim, D.B., Moon, S.Y. and Choe, W., “Escherichia Coli Deactivation Study Controlling the Atmospheric Pressure Plasma Discharge Conditions”, Current Applied Physics, 9(2009):625-628 Heise, M., Neff, W., Franken, O., Muranyi, P. and Wunderlich, J., “Sterilization of Polymer Foils with Dielectric Barrier Discharges at Atmospheric Pressure”, Plasma and Polymers, 9(2004):23-33 Hueso, J.L., Rico, V.J., Frías, J.E., Cotrino, J. and González-Elipe, A.R., “Ar＋ NO Microwave Plasma for Escherichia Coli Sterilization”, Journal of Physics D Applied Physics, 41(2008):092002.1-4 Hury, S., Vidal, D.R., Desor, F., Pelletier, J. and Lagarde, T., “A Parametric Study of the Destruction Efficiency of Bacillus Spores in Low Pressure Oxygen-Based Plasma”, Letters in Applied Microbiology, 26(1998):417-421 Ito, T., Yokoyama, H., Sato, T. and Ogata, T., “Influence of the Lens Effect in a Sample with Large Dielectric Constant in a Loop-Gap Resonator on the EPR Signal Intensity at 700 MHz”, Applied Magnetic Resonance, 21(2001): 97-103 Kim, J.G., Yousef, A.E. and Dave, S., “Application of Ozone for Enhancing the Microbiological Safety and Quality of Foods: A Review”, Journal of Food Protection, 62(1999):1071-1087 Kostov, K.G., Rocha, V., Koga-Ito, C.Y., Matos, B.M., Algatti, M.A., Honda, R.Y., Kayama, M.E. and Mota, R.P., “Bacterial Sterilizaiton by a Dielectric Barrier Discharge (DBD) in Air”, Surface and Coatings Technology, 204(2010):2954-2959 Laroussi, M. and Leipold, F., “Evaluation of the Roles of Reactive Species, Heat, and UV Radiation in the Inactivation of Bacterial Cell by Air Plasma at Atmospheric Pressure”, International Journal of Mass Spectrometry, 233(2004):81-86 Laroussi, M., Mendis, D.A., and Rosenberg, M., “Plasma Interaction with Microbes”, New Journal of Physics, 5(2003):41.1-41.10 Laroussi, M., Richardson, J.P., and Dobbs, F.C., “Effects of Nonequilibrium Atmospheric Pressure Plasmas on the Heterotrophic Pathways of Bacteria and on Their Cell Morphology”, Applied Physics Letters, 81(2002): 772-774 Lee, K., Paek, K.H., Ju, W.T. and Lee, Y., “Sterilization of Bacteria, Yeast, and Bacterial Endospores by Atmosphere-Pressure Cold Plasma Using Helium and Oxygen”, Journal of Microbiology, 44(2006):269-275 Lee, M.H., Park, B.J., Jin, S.C., Kim, D., Han, I., Kim, J., Hyun, S.O., Chung, K.H. and Park, J.C., “Removal and Sterilization of Biofilms and Planktonic Bacteria by Microwave-Induced Argon Plasma at Atmospheric pressure”, New Journal of Physics, 11(2009):115022 Lerouge, S., Wertheimer, M.R., Marchand, R., Tabrizian, M. and Yahia, L’H., “Effect of Gas Composition on Spore Mortality and Etching during Low-Pressure Plasma Sterilization”, Journal of Biomedical Materials Research, 51(2000):128-135 Liu, H., Chen, J., Yang, L. and Zhou, Y., “Long-Distance Oxygen Plasma Sterilization: Effects and Mechanisms”, Applied Surface Science, 254(2008):1815-1821 Lu, X.P., Jiang, Z.H., Xiong, Q., Tang, Z.Y. and Pan, Y., “A Single Electrode Room-Temperature Plasma Jet Device for Biomedical Applications”, Applied Physics Letters, 92(2008):151504.1-3 Ma, Y., Zhang, G.J., Shi, X.M., Xu, G.M. and Yang, Y., “Chemical Mechanism of Bacterial Inactivation Using Dielectric Barrier Discharge Plasma in Atmospheric Air”, IEEE Transactions on Plasma Science, 36(2008):1615-1620 Moisan, M., Barbeau, J., Crevier, M.C., Pelletier, J., Philip, N. and Saoudi, B., “Plasma Sterilization, Methods and Mechanisms”, Pure and Applied Chemistry, 74(2002):349-358 Moisan, M., Barbeau, J., Moreau, S., Pelletier, J., Tabrizian, M. and Yahia, L’H., “Low-Temperture Sterilization Using Gas Plasmas: A Review of the Experiments and an Analysis of the Inactivation Mechanisms”, International Journal of Pharmaceutics, 226(2001):1-21 Montie, T.C., Kelly-Wintenberg, K. and Reece Roth, J., “An Overview of Research Using the One Atmosphere Uniform Glow Discharge Plasma (OAUGDP) for Sterilization of Surfaces and Materials”, IEEE Transactions on Plasma Science, 28(2000):41-50 Moore, G., Griffith, C. and Peters, A., “Bactericidal Properties of Ozone and Its Potential Application as a Terminal Disinfectant”, Journal of Food Protection, 63(2000):1100-1106 Moreau, M., Orange, N. and Feuilloley, M.G.J., “Non-Thermal Plasma Technologies: New Tools for Bio-Decontamination”, Biotechnology Advances, 26(2008):610-617 Moreira, A.J., Mansano, R.D., Pinto, T.J.A., Ruas, R., Zambon, L.S., Silva, M.V. and Verdonck, P.B., “Sterilization by Oxygen Plasma”, Applied Surface Science, 235(2004):151-155 Morgan, N. N., “Atmospheric Pressure Dielectric Barrier Discharge Chemical and Biological Applications”, International Journal of the Physical Sciences, 4(2009):885-892 Park, B.J., Takatori, K., Lee, M.H., Han, D.W., Woo, Y.I., Son, H.J., Kim, J.K., Chung, K.H., Hyun, S.O. and Park, J.C., “Escherichia Coli Sterilization and Lipopolysaccharide Inactivation Using Microwave-Induced Argon Plasma at Atmospheric Pressure”, Surface & Coatings Technology, 201(2007):5738- 5741 Pauling, L., “The Nature of the Chemical Bond. IV. The Energy of Single Bonds and the Relative Electronegativity of Atoms”, Journal of the American Chemical Society, 54(1932):3570-3582 Pointu, A.M., Ricard, A., Dodet, B., Odic, E., Larbre, J. and Ganciu, M., “Production of Active Species in N2-O2 Flowing Post-Discharge at Atmospheric Pressure for Sterilization”, Journal of Physics D Applied Physics, 38(2005):1905-1909 Saadat, H., “Power System Analysis”, 2nd edition, McGrawHill Companies, 2004 Salamitou, S., Kirkpatrick, M.J., Ly, H.M., Leblon, G., Odic, E. and DuBow, M.S., “Augmented Survival of Bacteria within Biofilms to Exposure to an Atmospheric Pressure Non-Thermal Plasma Source”, Biotechnology, 8(2009):228-234 Schütze, A., Jeong, J.Y., Babayan, S.E., Park, J., Selwyn, G.S. and Hicks, R.F., “The Atmospheric-Pressure Plasma Jet: A Review and Comparison to Other Plasma Sources”, IEEE Transactions on Plasma Science, 26(1998):1685-1694 Sharma, A., Pruden, A., Stan, O. and Collins, G.J., “Bacterial Inactivation Using an RF-Powered Atmospheric Pressure Plasma”, IEEE Transactions on Plasma Science, 34(2006):1290-1296 Sladek, R.E.J., Filoche, S.K., Sissons, C.H. and Stoffels, E., “Treatment of Streptococcus Mutans Biofilms with a Nonthermal Atmospheric Plasma”, Letters in Applied Microbiology, 45(2007):318-323 Uhm, H.S., Lim, J.P. and Li, S.Z., “Sterilization of Bacterial Endospores by an Atmospheric-pressure Argon Plasma Jet”, Applied Physics Letters, 90(2007): 261501.1-3 Viscoveanu, D., Popescu, S., Ohtsu, Y. and Fujita, H., “Competing Inactivation Agent for Bacterial Spores in Radio-Frequency Oxygen Plasmas”, Plasma Process and Polymers, 5(2008):350-358 Vollrath, K. and Thomer, G. (ed.), “Kurzzeitphysik”, Springer, Wien, 1967 Xu, G., Zhang, G., Shi, X., Ma, Y., Wang, N. and Li, Y., “Bacteria Inactivation Using DBD Plasma Jet in Atmoshperic Pressure Argon”, Plasma Science and Technology, 11(2009):83-88 Yamashita, R., Takahashi, T. and Oda, T., “Humidity Effect on Non-Thermal Plasma Processing for VOCs Decomposition”, Proceedings of the 1996 Industry Applications Society Annual Meeting, San Diego, CA, Oct. 6-10, 1996 Yu, Q.S., Huang, C., Hsieh, F.H., Huff, H. and Duan, Y., “Bacterial Inactivation Using a Low-Temperature Atmospheric Plasma Brush Sustained with Argon Gas”, Journal of Biomedical Materials Research Part B: Applied Biometerials, 80(2007):211-219 王玉純，「臭氧對生物氣膠殺菌效率之評估」，國立台灣大學環境衛生研究所，2001 台灣醫院感染管制學會，「醫療物品之消毒與滅菌」，2005 何政昌，「常壓電漿技術之研究」，國立成功大學化學工程研究所，2001 李灝銘，「以低溫電漿去除揮發性有機物之研究」，國立中央大學環境工程研究所，2001 林明瀅，「實驗室消毒與滅菌原理及應用（含標準操作程序）」，台北榮民總醫院感染管制室，2007 林愛紅，饒健，秦彥鞏，黃惠英，「臭氧殺菌效果的影響因素分析」，湖北預防醫學雜念2002年第13卷第6期 施明良，「六氟化硫於氧化及還原高週波電漿中之反應機制」，國立成功大學環境工程學系，2003 洪啟強，「電子學¬¬¬¬─機械、工管科適用」，二版，全華科技圖書股份有限公司，2001 張家豪，魏鴻文，翁政輝，柳克強，李安平，寇崇善，吳敏文，曾錦清，蔡文發，鄭國川，「電漿源原理與應用之介紹」，物理雙月刊2006年第28卷第2期 陳宏凱，「氮氣光譜之研究Ⅱ：C3Πu－X1Σg＋及a1Σg－X1Σg＋系統」，國立中央大學化學研究所，2001 楊美桂，「微生物學」，藝軒圖書出版社，2003 楊黃捷，「利用原子力顯微鏡研究紫外光殺菌之衰亡機制」，國立台灣師範大學物理學系，2009 環境檢驗所，「NIEA E203.55B 水中總菌落數檢測方法－塗抹法」，2007 行政院衛生署，「部分感染性醫療廢棄物滅菌處理標準及相關規定」，2001
指導教授	張木彬(Moo-been Chang)	審核日期	2011-7-20
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