應用歸零原理和極化對比技術之影像式波導共振生物感測器

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姓名

張富程(Fu-chen Chang) 查詢紙本館藏

畢業系所

光電科學與工程學系

論文名稱

應用歸零原理和極化對比技術之影像式波導共振生物感測器
(Application of Nulling Theory and Polarization Contrast for Imaging Type Guided-Mode Resonance Biosensor)

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摘要(中)

本研究在研發一新型影像式波導共振生物感測器。隨著科技的發展，人們越來越注重疾病的預防，也是生物感測器越來越蓬勃發展的主要原因。從早期的一維單點量測生物感測器慢慢演化至現在二維多點影像式生物感測器，中間的技術不斷的進步及突破。其中又以光學式的生物感測器較為容易達到影像式生物感測器的目標。本研究以波導共振生物感測器為基礎，加上極化對比技術和歸零原理技術設計出高對比度低雜訊的即時偵測影像式生物感測器，並且可針對不同的待測物來調整感測範圍。
　　波導共振元件本身具有濾波的性質，除了在特定的共振波長下使穿透元件之光的穿透率為零，也會使穿透光的相位產生變化。因此當光通過此元件時會有偏振態的改變，再配合了歸零原理利用偏振片架構來改變穿透元件之穿透光的偏振態達到歸零的效果。最後利用極化對比技術降低CCD成像雜訊。本研究其理想檢測極限為4.865×10-5 RIU，線性檢測區間為1.339 RIU至1.3342 RIU。實際檢測極限達到1.008×10-4 RIU，線性檢測區間為1.336 RIU至1.3340 RIU，此系統可量測折射率1.33至1.365的鹽水，並且可藉由調整GMR共振波長，配合不同折射率的待測物設計最佳檢測曲線。

摘要(英)

In this thesis we develop a novel imaging type guided-mode resonance (GMR) biosensor. In the past few decades, biosensor was developed quickly and successfully. There are many different types of biosensors such as electrochemical biosensor, optical biosensor, and biocatalytic biosensor. Especially in optical biosensor, the imaging type biosensors such as multi-point imaging biosensor was developed rapidly because it offers the properties of quantitative bimolecular information and it is useful for direct visualization of cell behavior.
　　This thesis is based on GMR biosensor and combining polarization contrast with nulling theory for designing the high-contrast and low-noise real-time imaging biosensor system. The system can also be designed different sensing area for different objects. The ideal limit of detection (LOD) of this system is 4.865×10-5 RIU and the linear operating range is from 1.339 RIU to1.3342 RIU. The actual LOD of the system is 1.008×10-4 RIU and the linear operating area from 1.336 RIU to1.3340RIU. The sensing range of this system is from 1.330 RIU to 1.365 RIU; moreover, the sensitivity can be designable for different objects by modulating the resonated wavelength of GMR.

關鍵字(中)

★ 生物感測器
★ 極化對比
★ 歸零原理
★ 影像式感測器
★ 波導共振

關鍵字(英)

★ biosensor
★ GMR
★ nulling theory
★ polarization contrast

論文目次

目錄
摘要 I
ABSTRACT II
致謝 III
目錄 IV
圖目錄 VI
第一章序論 1
1-1生物感測器 1
1-1.1生物感測器簡介 1
1-1.2生物感測器起源極發展 2
1-1.3生物感測器分類 4
1-2影像式生物感測器 6
1-3 GMR應用在生物感測器 10
1-4研究動機 13
第二章影像式波導共振生物感測器原理 14
2-1波導共振元件理論 14
2-1.1光波導原理繞射 14
2-1.2光柵原理 16
2-1.3波導共振原理 17
2-1.4 TE和TM穿透光之相位差 19
2-2歸零原理(nulling) 20
2-3具歸零原理架構之影像式波導共振感測器操作原理 22
第三章模擬、製程與量測 28
3-1影像式波導共振感測器之模擬 28
3-1.1 GMR之模擬 28
3-1.2 歸零技術(nulling)之模擬 33
3-2 單晶片介紹波導共振元件之製程 35
3-2.1晶片製程 35
3-2.2微流道系統 37
3-3 影像式波導共振感測器之量測 39
第四章實驗結果與討論 44
4-1 晶片製程 44
4-2光學模擬 45
4-3鹽水濃度之量測 58
第五章結論與未來展望 63
5-1 結論 63
5-2未來展望 64
第六章参考文獻 65

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指導教授

張正陽(Jenq-Yang Chang)

審核日期

2011-10-12

推文