探討以離子液體[Hmim][PF6]進行萃取發酵來提升Clostridium acetobutylicum 產丁醇之研究

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吳孟隆(Meng-Long Wu) 查詢紙本館藏

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化學工程與材料工程學系

論文名稱

探討以離子液體[Hmim][PF6]進行萃取發酵來提升Clostridium acetobutylicum 產丁醇之研究

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摘要(中)

本研究是利用疏水性離子液體[Hmim][PF6]對Clostridium acetobutylicum進行萃取式發酵，以降低發酵液中丁醇濃度來改善產物抑制效應問題，並且搭配活性碳吸附來解決離子液體所造成的毒性問題。以1:1的比例進行萃取發酵時，可降低發酵液中丁醇濃度2.2g/L，而離子液體可經由水洗後真空濃縮來重複利用，但菌體受到毒性的影響則無法繼續生產丁醇。新鮮培養液若接觸離子液體後，用於菌種培養時菌體完全無法生長；但若由活性碳以1 : 10 (w/v) 吸附5分鐘後，用於培養菌體可達到控制組約70 %的丁醇產量，且發酵液經由活性碳吸附亦可降低發酵液中丁醇濃度。但此操作條件應用於萃取發酵後雖移除了2.99 g/L丁醇，但菌體所受的毒性抑制效果仍未見起色。因此未來應尋找較佳的操作條件以達到提昇產量的效果。

摘要(英)

In this research, the hydrophobic ionic liquid, [Hmim][PF6] was applied to the extractive fermentation of Clostridium acetobutylicum to remove butanol from the broth. The toxic ionic liquid remained in the broth was adsorbed by activated carbon. During the extraction operation, 2.2 g/L of butanol was removed from the broth. The production of butanol was stopped after the extraction due to the toxicity of residual ionic liquid. C. acetobutylicum cannot grow in the medium which had contact with the ionic liquid. After adsorption by 1 : 10 (w/v) activated carbon for 5 minutes, the production of butanol by C. acetobutylicum can reach 70% of the amount of control experiment . The fermentation after extraction and adsorption can remove 2.99 g/L of butanol in the broth. But the butanol production cannot be enhanced by current operation conditions. It means that more operation conditions should be discussed to find the optimal way to improve the butanol production.

關鍵字(中)

★ 丁醇
★ 離子液體
★ 萃取發酵
★ 活性碳

關鍵字(英)

★ Clostridium acetobutylicum
★ [Hmim][PF6]

論文目次

第一章緒論 1
1-1 研究動機 1
1-2 研究目的 2
第二章文獻回顧 3
2-1 生質能 3
2-2 丁醇 3
2-2-1 丁醇基本性質 3
2-2-2 丁醇生產方式 4
2-2-3 丁醇的應用 5
2-3 ABE發酵 6
2-3-1 ABE發酵介紹 6
2-3-2 ABE發酵製程改良法 7
2-4 Clostridium 5
2-5 離子液體(Ionic Liquids，ILs) 8
2-5-1 離子液體發展動機 14
2-5-2 離子液體定義 14
2-5-3 離子液體的發展 16
2-5-4 離子液體的性質 16
2-5-5 離子液體的應用 17
2-5-6 離子液體毒性研究 18
2-6 活性碳(Activated carbon，AC) 19
2-6-1 活性碳製造 20
2-6-2 活性碳分類 21
2-6-3 活性碳之物化特性 22
2-6-4 活性碳之應用 25
2-7 吸附基本原理 25
2-7-1 吸附現象 25
2-7-2 吸附種類 27
第三章材料與方法 30
3-1 實驗材料 30
3-1-1 微生物 30
3-1-2 培養基組成 31
3-1-3 實驗藥品 32
3-1-4 實驗儀器及其他設備 34
3-2 實驗設計與方法 36
3-2-1 菌種保存 36
3-2-2 接種菌體培養 37
3-2-3 攪拌式發酵槽 38
3-2-4 離子液體[Hmim][PF6]製備 39
3-2-5 活性碳降低離子液體毒性測試 41
3-3 分析方法 41
3-3-1菌體量的測定 41
3-3-2 葡萄糖殘量的測定 42
3-3-3 ABE的分析 43
第四章結果與討論 45
4-1離子液體合成品測試 45
4-2 離子液體重複萃取利用測試 46
4-3 ABE發酵實驗控制組 47
4-4 離子液體萃取發酵實驗 48
4-5 利用活性碳吸附來降低離子液體毒性之可行性測試 49
4-5-1 探討活性碳添加比例對降低離子液體毒性之影響 51
4-5-2 探討活性碳吸附時間對降低離子液體毒性之影響 54
4-6 活性碳吸附對發酵之影響 57
4-7萃取發酵後以活性碳吸附實驗 59
第五章結論與建議 60
5-1 結論 60
5-2 建議 61
第六章參考文獻 62

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指導教授

徐敬衡(Chin-Hang Shu)

審核日期

2013-8-28

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