博碩士論文 100324031 完整後設資料紀錄

DC 欄位 語言
DC.contributor化學工程與材料工程學系zh_TW
DC.creator吳孟隆zh_TW
DC.creatorMeng-Long Wuen_US
dc.date.accessioned2013-8-28T07:39:07Z
dc.date.available2013-8-28T07:39:07Z
dc.date.issued2013
dc.identifier.urihttp://ir.lib.ncu.edu.tw:88/thesis/view_etd.asp?URN=100324031
dc.contributor.department化學工程與材料工程學系zh_TW
DC.description國立中央大學zh_TW
DC.descriptionNational Central Universityen_US
dc.description.abstract本研究是利用疏水性離子液體[Hmim][PF6]對Clostridium acetobutylicum進行萃取式發酵,以降低發酵液中丁醇濃度來改善產物抑制效應問題,並且搭配活性碳吸附來解決離子液體所造成的毒性問題。以1:1的比例進行萃取發酵時,可降低發酵液中丁醇濃度2.2g/L,而離子液體可經由水洗後真空濃縮來重複利用,但菌體受到毒性的影響則無法繼續生產丁醇。新鮮培養液若接觸離子液體後,用於菌種培養時菌體完全無法生長;但若由活性碳以1 : 10 (w/v) 吸附5分鐘後,用於培養菌體可達到控制組約70 %的丁醇產量,且發酵液經由活性碳吸附亦可降低發酵液中丁醇濃度。但此操作條件應用於萃取發酵後雖移除了2.99 g/L丁醇,但菌體所受的毒性抑制效果仍未見起色。因此未來應尋找較佳的操作條件以達到提昇產量的效果。zh_TW
dc.description.abstractIn this research, the hydrophobic ionic liquid, [Hmim][PF6] was applied to the extractive fermentation of Clostridium acetobutylicum to remove butanol from the broth. The toxic ionic liquid remained in the broth was adsorbed by activated carbon. During the extraction operation, 2.2 g/L of butanol was removed from the broth. The production of butanol was stopped after the extraction due to the toxicity of residual ionic liquid. C. acetobutylicum cannot grow in the medium which had contact with the ionic liquid. After adsorption by 1 : 10 (w/v) activated carbon for 5 minutes, the production of butanol by C. acetobutylicum can reach 70% of the amount of control experiment . The fermentation after extraction and adsorption can remove 2.99 g/L of butanol in the broth. But the butanol production cannot be enhanced by current operation conditions. It means that more operation conditions should be discussed to find the optimal way to improve the butanol production.en_US
DC.subject丁醇zh_TW
DC.subject離子液體zh_TW
DC.subject萃取發酵zh_TW
DC.subject活性碳zh_TW
DC.subjectClostridium acetobutylicumen_US
DC.subject[Hmim][PF6]en_US
DC.title探討以離子液體[Hmim][PF6]進行萃取發酵來提升Clostridium acetobutylicum 產丁醇之研究zh_TW
dc.language.isozh-TWzh-TW
DC.type博碩士論文zh_TW
DC.typethesisen_US
DC.publisherNational Central Universityen_US

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