The purpose of this study is to design a mechanical force to stimulate human bladder cancer cell microfluidic chips, mechanical forces, including the shear stress and the normal stress. The microfluidic chip has three channel, each channel has three cultures area were to do in cell culture, three shear stress is applied to each flow path through Syringe pump and control valve, respectively. And then application of normal stress on cultures area.
Human bladder carcinoma (TCC) cultured in the area of the microfluidic chip, the cells were applied to three kinds of shear stress stimulation 10 minutes (0.0018 dyne / cm2,0.044 dyne / cm2,0.66 dyne / cm2.), and then the each of three shear stress applied normal stress stimulation one hour (0KPa, 10KPa, 100KPa), then cultured in an incubator for 24 hours, subject to different stimulation parameters for each group cells were photographed, the number of stimulation cells before and after.
Experimental results show that by the cell, the cell number of the maximum shear stress stimulation downward trend; 0KPa and 10KPa normal stress stimulation on the number showed positive growth, the number of cells to 100KPa presented negative growth.