DC 欄位 值 語言 DC.contributor 生命科學系 zh_TW DC.creator 陳毅 zh_TW DC.creator Yi Chen en_US dc.date.accessioned 2017-1-25T07:39:07Z dc.date.available 2017-1-25T07:39:07Z dc.date.issued 2017 dc.identifier.uri http://ir.lib.ncu.edu.tw:88/thesis/view_etd.asp?URN=102224003 dc.contributor.department 生命科學系 zh_TW DC.description 國立中央大學 zh_TW DC.description National Central University en_US dc.description.abstract Forkhead box class O (FoxO)家族基因包含有FoxO1、FoxO3、FoxO4和FoxO6四種,過去有許多文獻分別指出各FoxO基因在細胞內調控了包括分化、葡萄醣代謝、DNA修復和細胞凋亡在內的許多功能。在本實驗中我們使用了3T3-L1前脂肪細胞分化為脂肪細胞的系統,透過處理Dexamethasone、Insulin和3-isobutyl-1-methylxanthine等3種藥物所組成之分化劑,促使細胞進行為期14天的分化。在分化的過程中,細胞的數目有明顯的增加,而細胞內會有三酸甘油脂的堆積。在我們的實驗中發現,相較於沒有處理EGCG的組別,在分化期間處理EGCG會使細胞內油滴的數量減少。當沒有處理EGCG時,細胞的FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量在分化過程的第2~6天之間會有增加的趨勢,而在第6~14天之間的表現量則會有降低的趨勢。在14天的分化期間,EGCG會大幅降低FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量。此外ATF-3的mRNA表現量則會有增加的趨勢,其表現量並不會受到EGCG的影響。這些實驗結果顯示EGCG會透過調節FoxO的表現量去影響3T3-L1前脂肪細胞的分化。 zh_TW dc.description.abstract The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels. en_US DC.subject 脂肪細胞 zh_TW DC.subject 兒茶素 zh_TW DC.subject 細胞分化 zh_TW DC.subject FoxO en_US DC.subject EGCG en_US DC.subject adipocyte en_US DC.subject differentiation en_US DC.subject ATF-3 en_US DC.title 綠茶表沒食子酸酯型兒茶素酸酯在3T3-L1脂肪細胞分化過程中調節FoxO家族基因的表現 zh_TW dc.language.iso zh-TW zh-TW DC.title Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells en_US DC.type 博碩士論文 zh_TW DC.type thesis en_US DC.publisher National Central University en_US