博碩士論文 102224003 完整後設資料紀錄

DC 欄位 語言
DC.contributor生命科學系zh_TW
DC.creator陳毅zh_TW
DC.creatorYi Chenen_US
dc.date.accessioned2017-1-25T07:39:07Z
dc.date.available2017-1-25T07:39:07Z
dc.date.issued2017
dc.identifier.urihttp://ir.lib.ncu.edu.tw:88/thesis/view_etd.asp?URN=102224003
dc.contributor.department生命科學系zh_TW
DC.description國立中央大學zh_TW
DC.descriptionNational Central Universityen_US
dc.description.abstractForkhead box class O (FoxO)家族基因包含有FoxO1、FoxO3、FoxO4和FoxO6四種,過去有許多文獻分別指出各FoxO基因在細胞內調控了包括分化、葡萄醣代謝、DNA修復和細胞凋亡在內的許多功能。在本實驗中我們使用了3T3-L1前脂肪細胞分化為脂肪細胞的系統,透過處理Dexamethasone、Insulin和3-isobutyl-1-methylxanthine等3種藥物所組成之分化劑,促使細胞進行為期14天的分化。在分化的過程中,細胞的數目有明顯的增加,而細胞內會有三酸甘油脂的堆積。在我們的實驗中發現,相較於沒有處理EGCG的組別,在分化期間處理EGCG會使細胞內油滴的數量減少。當沒有處理EGCG時,細胞的FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量在分化過程的第2~6天之間會有增加的趨勢,而在第6~14天之間的表現量則會有降低的趨勢。在14天的分化期間,EGCG會大幅降低FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量。此外ATF-3的mRNA表現量則會有增加的趨勢,其表現量並不會受到EGCG的影響。這些實驗結果顯示EGCG會透過調節FoxO的表現量去影響3T3-L1前脂肪細胞的分化。 zh_TW
dc.description.abstractThe forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels.en_US
DC.subject脂肪細胞zh_TW
DC.subject兒茶素zh_TW
DC.subject細胞分化zh_TW
DC.subjectFoxOen_US
DC.subjectEGCGen_US
DC.subjectadipocyteen_US
DC.subjectdifferentiationen_US
DC.subjectATF-3en_US
DC.title綠茶表沒食子酸酯型兒茶素酸酯在3T3-L1脂肪細胞分化過程中調節FoxO家族基因的表現zh_TW
dc.language.isozh-TWzh-TW
DC.titleGreen tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cellsen_US
DC.type博碩士論文zh_TW
DC.typethesisen_US
DC.publisherNational Central Universityen_US

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