| dc.description.abstract | Soot particle is one of the indicators of traffic pollution. It can contain organic, inorganic or even toxic substances easier due to its irregular structure. Exposure to soot particle significantly increased the risk of lung cancer. In recent years, many studies investigate the health effects after exposure to soot particles, but for the charged particles is not clear understanding yet. Therefore, this study establish a stable soot particle exposure system and study the biological responses of mouse after exposing to the charged soot particles. BALB/c female mice were exposed to soot particles into positively and negatively charged groups, each have four conditions (high concentration, low concentration, electrical and control group), and exposed for eight hours a day and last for a week. Soot particle size were select 70 nm by Differential Mobility Analyze (DMA) and confirm particle size by Scanning Mobility Particle Sizer (SMPS), Nanoparticle Surface Area Monitor (NSAM) measure the surface area of the particles deposited in the lungs, Aerosol Electrometer (AE) confirm the electric charge of particles, Aethalometer (AE31) and Optical Transmissometer (OT21) measure the concentration of soot particle. At the same time, filter were collect soot particle for composition and structure analysis. Based on the above instruments, they can monitor the stability of the system and the compare the accuracy of the measurement data. After exposure, we collected lung tissue, serum and bronchoalveolar lavage fluid of mouse to study the biological responses (BALF-Total Protein、BALF-LDH、Serum-IL-6、Lung-8-Isoprostane、Lung-Caspase-3、Lung-BPDE). The levels of BALF-LDH, Lung 8-Isoprostane and Serum IL-6 are well linearly correlated to BC mass concentration, lung deposition surface area concentration and charge current. Especially for BALF-LDH, the correlations are almost perfect linear. Furthermore, the responses for negative charged soot particles are significantly lower than both neutral and positive charge particles (especially in low exposure dosage range). | en_US |