博碩士論文 105326020 完整後設資料紀錄

DC 欄位 語言
DC.contributor環境工程研究所zh_TW
DC.creator張智聖zh_TW
DC.creatorChih-Sheng Changen_US
dc.date.accessioned2019-7-29T07:39:07Z
dc.date.available2019-7-29T07:39:07Z
dc.date.issued2019
dc.identifier.urihttp://ir.lib.ncu.edu.tw:88/thesis/view_etd.asp?URN=105326020
dc.contributor.department環境工程研究所zh_TW
DC.description國立中央大學zh_TW
DC.descriptionNational Central Universityen_US
dc.description.abstract近年來由於氣候變遷及人口快速上升,導致乾淨且可利用的水資源越來越稀少,因此污水回收再利用的需求及發展漸漸受到重視,未來也希望運用在用水量較多的農業灌溉,甚至是飲用水,但前提是再利用的水質需受到嚴格控管,這包括過往較常被忽略的生物性污染物,特別是抗生素抗性基因(antibiotic resistance genes, ARGs)與抗生素抗性菌(antibiotic resistance bacteria, ARB)。有鑒於污水處理廠已被認定為是原核生物獲取與交換抗性基因的潛在熱區,以及為了避免再生水中的抗生素抗藥性成為公共安全衛生和環境污染問題,本研究利用傳統細胞培養與qPCR基因定量等技術,並佐以參數彼此間相關性的統計分析,調查ARB與ARGs於污水處理單元的流佈,以期瞭解並掌握ARB與ARGs的動態變化、甚至預測其在污水處理程序過程的表現。調查結果顯示:(1)使用MBR處理後對於ARB (從5.2log – 6.2log CFU/mL降低至1.1log – 3.4log CFU/mL)、ARGs (從5.4log – 13.1log copy/L降低至0.6log – 8.2log copy/L)和class 1 integron (從11.5log – 12.9log copy/L降低至3.8log – 3.9log copy/L)具有顯著的絕對濃度去除效果;(2) ARB的絕對濃度經加氯或UV消毒後顯著下降(p < 0.05) (Acl廠:從4.3log – 6.1log CFU/mL降低至1.2log – 3.7log CFU/mL;Cuv廠:從3.0log – 4.6log CFU/mL降低至1.3log – 3.2log CFU/mL),且兩個處理廠的消毒處理前後變化率具顯著差異(p < 0.05);(3)有無加氯消毒或UV消毒對於ARGs和class 1 integron的絕對濃度前後變化率不具顯著差異(p > 0.05) (Acl廠:從5.5log – 11.2log copy/L降低至5.5log – 11.1log copy/L;Bmbr廠:從0.6log – 8.2log copy/L降低至1.6log – 5.6log copy/L;Cuv廠:從3.5log – 7.6log copy/L降低至3.4log – 8.5log copy/L);(4)經過MBR處理後對於ARGs和class 1 integron相對濃度有較好的處理效率;(5)經過加氯消毒和UV消毒對於ARGs的相對濃度的變化率不具顯著差異(p > 0.05),且加氯消毒對於整體ARGs和class 1 integron的相對濃度變化率呈現下降、UV消毒對於整體ARGs和class 1 integron呈現濃度上升;(6)冗餘分析顯示COD、總磷、總氮、總凱氏氮和pH與ARGs和class 1 integron具有相關係,與硝酸鹽氮、亞硝酸鹽氮、溶氧濃度和溫度的相關性較小。這些調查結果說明當處理廠使用MBR時可以有良好的 ARB和ARGs去除效果,但是對於目前常用的加氯和UV消毒方法則可能需要調整其操作參數或使用更有效的處理技術(如:臭氧、Fenton)以避免回收水中含有較多的 ARGs;此外,由相關性分析所得的結果表明ARGs在廢水處理程序的多寡似乎與一般的厭氧參數有較強的關聯性,對此值得後續加以追蹤與確認。zh_TW
dc.description.abstractDue to climate change and population explosion, shortage of clean and safe water resources is becoming an increasingly serious problem worldwide. Development of reuse of domestic wastewater could be one of the solutions. Ultimately, it is aimed to use recycled wastewater in agricultural irrigation and even in drinking water. To meet this goal, the water quality of recycled wastewater has to be demanded absolutely safe at every level, both chemically and biologically. Given that wastewater treatment plants (WWTPs) have been considered hot spots for the proliferation of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), investigating the dynamics of ARB and ARG levels during the wastewater treatment process and their association with other generic water quality parameters will be one step closer to the goal of wastewater reuse. In this study, influents and effluents of each operating unit of three WWTPs were collected in the Fall and Spring. In addition to generic water quality parameters, the collected samples were analyzed for the abundance of ARB ARGs via cell incubation and qPCR methods. Results show that: (1) After MBR treatment, the absolute abundances of ARB (from 5.2log – 6.2log CFU/mL to 1.1log – 3.4log CFU/mL), ARGs (from 5.4log – 13.1log copy/L to 0.6log – 8.2log copy/L) and class 1 integron (from 11.5log – 12.9log copy/L to 3.8log – 3.9log copy/L) are substantively reduced; (2) The absolute abundance of ARB is significantly decreased after chlorination or UV disinfection (p < 0.05) (plant Acl: from 4.3log – 6.1log CFU/mL to as low as 1.2log – 3.7log CFU/ml; plant Cuv: from 3.0log – 4.6log CFU/mL and reduced to 1.3log – 3.2log CFU/mL), and the removal rates between the two plants are significantly different (p < 0.05); (3) Neither chlorination nor UV disinfection results in a significant decrease in the absolute abundance of ARG and intI (p > 0.05) (plant Acl: 5.5log – 11.2log copy/L reduced to 5.5log – 11.1log copy/L, plant Bmbr: 0.6log – 8.2log copy/L reduced to 1.6log – 5.6log copy/L, plant Cuv: 3.5log – 7.6log copy/L reduced to 3.4log – 8.5log copy/L); (4) higher removal efficiency of ARGs and class 1 integron relative abundance is observed in MBR treatment; (5) chlorination and UV disinfection do not significantly affect relative abundances of ARGs; (6) RDA show that relative abundances of ARGs and class 1 integron correlate well with COD, total phosphorus, total nitrogen, total Kjeldahl nitrogen concentration and pH, and little with nitrate nitrogen, nitrite nitrogen, dissolved oxygen concentration and temperature. Together, these results indicate that WWTPs implementing MRB can have better ARB and ARGs removal efficiency, and current disinfection units that use chlorination and UV may need to adjust operating parameters or to adopt more effective technologies (e.g., ozonation, Fenton oxidation) to reduce the risk of higher levels of ARGs appearing in the recovered water. In addition, the strong association between ARGs and certain anaerobic parameters suggests that future investigation on this regard is warranted.en_US
DC.subject都市污水處理廠zh_TW
DC.subject抗生素抗性菌zh_TW
DC.subject抗生素抗性基因zh_TW
DC.subject消毒程序zh_TW
DC.subjectDomestic treatment planten_US
DC.subjectantibiotic resistance bacteriaen_US
DC.subjectantibiotic resistance geneen_US
DC.subjectdisinfection procedureen_US
DC.title抗生素抗性菌與抗性基因在污水處理程序中的動態變化zh_TW
dc.language.isozh-TWzh-TW
DC.titleDynamics of antibiotic resistant bacteria and genes in the domestic wastewater treatment processen_US
DC.type博碩士論文zh_TW
DC.typethesisen_US
DC.publisherNational Central Universityen_US

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